Condensin Relocalization from Centromeres to Chromosome Arms Promotes Top2 Recruitment during Anaphase
| العنوان: | Condensin Relocalization from Centromeres to Chromosome Arms Promotes Top2 Recruitment during Anaphase |
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| المؤلفون: | Joanne Leonard, Takashi Sutani, Katsuhiko Shirahige, Adam Jarmuz, Nicholas Sen, Raul Torres, Luis Aragón |
| المصدر: | Cell Reports Cell Reports, Vol 13, Iss 11, Pp 2336-2344 (2015) |
| بيانات النشر: | Cell Press, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Saccharomyces cerevisiae Proteins, Condensin, Centromere, macromolecular substances, Saccharomyces cerevisiae, Biology, General Biochemistry, Genetics and Molecular Biology, Mitotic chromosome condensation, Chromosome segregation, Report, Chromosome Segregation, Sister chromatids, YEAST, SEGREGATION, SISTER CHROMATIDS, PHOSPHORYLATION, lcsh:QH301-705.5, Anaphase, Genetics, Adenosine Triphosphatases, Science & Technology, COMPLEX, Kinetochore, 13S CONDENSIN, DECATENATION, DNA TOPOISOMERASE-II, Cell Biology, DNA, MITOTIC CHROMOSOMES, DNA-Binding Proteins, Anaphase lag, DNA Topoisomerases, Type II, lcsh:Biology (General), MITOSIS, Multiprotein Complexes, biology.protein, Chromosomes, Fungal, Life Sciences & Biomedicine, Protein Binding |
| الوصف: | Summary Condensin is a conserved chromosomal complex necessary to promote mitotic chromosome condensation and sister chromatid resolution during anaphase. Here, we report that yeast condensin binds to replicated centromere regions. We show that centromeric condensin relocalizes to chromosome arms as cells undergo anaphase segregation. We find that condensin relocalization is initiated immediately after the bipolar attachment of sister kinetochores to spindles and requires Polo kinase activity. Moreover, condensin localization during anaphase involves a higher binding rate on DNA and temporally overlaps with condensin’s DNA overwinding activity. Finally, we demonstrate that topoisomerase 2 (Top2) is also recruited to chromosome arms during anaphase in a condensin-dependent manner. Our results uncover a functional relation between condensin and Top2 during anaphase to mediate chromosome segregation. Graphical Abstract Highlights • Condensin recruitment to centromeric regions requires DNA replication • Centromeric condensin spreads to chromosome arms during anaphase • Condensin promotes recruitment of Top2 during anaphase • Condensin localization requires Polo kinase and correlates with DNA overwinding Chromosome condensation requires condensin and topoisomerase 2 (Top2) and is necessary for segregation. Leonard et al. show that condensin localizes to yeast centromeres following DNA replication and spreads to chromosome arms during anaphase when Top2 is also recruited. Condensin and Top2 activities are coordinated to shape mitotic chromosomes. |
| اللغة: | English |
| تدمد: | 2211-1247 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2c1a7b6d2dfb95b7dfeee45a0a02779Test http://europepmc.org/articles/PMC4695335Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f2c1a7b6d2dfb95b7dfeee45a0a02779 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22111247 |
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