دورية أكاديمية
Generation of Tumor Antigen-Specific iPSC-Derived Thymic Emigrants Using a 3D Thymic Culture System.
| العنوان: | Generation of Tumor Antigen-Specific iPSC-Derived Thymic Emigrants Using a 3D Thymic Culture System. |
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| المؤلفون: | Vizcardo, Raul, Klemen, Nicholas D, Islam, S M Rafiqul, Gurusamy, Devikala, Tamaoki, Naritaka, Yamada, Daisuke, Koseki, Haruhiko, Kidder, Benjamin L, Yu, Zhiya, Jia, Li, Henning, Amanda N, Good, Meghan L, Bosch-Marce, Marta, Maeda, Takuya, Liu, Chengyu, Abdullaev, Zied, Pack, Svetlana, Palmer, Douglas C, Stroncek, David F, Ito, Fumito, Flomerfelt, Francis A, Kruhlak, Michael J, Restifo, Nicholas P |
| المصدر: | Cell Rep ; ISSN:2211-1247 ; Volume:22 ; Issue:12 |
| بيانات النشر: | Elsevier Science |
| سنة النشر: | 2018 |
| المجموعة: | PubMed Central (PMC) |
| مصطلحات موضوعية: | 3D culture, T cell differentiation, adoptive cell transfer, fetal thymus organ culture, iPSC differentiation, immunotherapy, naïve T cell, recent rhymic emigrants, thymopoiesis, tumor antigen specific T cell |
| الوصف: | Induced pluripotent stem cell (iPSC)-derived T cells may provide future therapies for cancer patients, but those generated by current methods, such as the OP9/DLL1 system, have shown abnormalities that pose major barriers for clinical translation. Our data indicate that these iPSC-derived CD8 single-positive T cells are more like CD4+CD8+ double-positive T cells than mature naive T cells because they display phenotypic markers of developmental arrest and an innate-like phenotype after stimulation. We developed a 3D thymic culture system to avoid these aberrant developmental fates, generating a homogeneous subset of CD8αβ+ antigen-specific T cells, designated iPSC-derived thymic emigrants (iTEs). iTEs exhibit phenotypic and functional similarities to naive T cells both in vitro and in vivo, including the capacity for expansion, memory formation, and tumor suppression. These data illustrate the limitations of current methods and provide a tool to develop the next generation of iPSC-based antigen-specific immunotherapies. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://doi.org/10.1016/j.celrep.2018.02.087Test; https://pubmed.ncbi.nlm.nih.gov/29562175Test; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5930030Test/ |
| DOI: | 10.1016/j.celrep.2018.02.087 |
| الإتاحة: | https://doi.org/10.1016/j.celrep.2018.02.087Test https://pubmed.ncbi.nlm.nih.gov/29562175Test https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5930030Test/ |
| حقوق: | Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved. |
| رقم الانضمام: | edsbas.DDC18B4D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.celrep.2018.02.087 |
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