Transgelin is a TGFβ-inducible gene that regulates osteoblastic and adipogenic differentiation of human skeletal stem cells through actin cytoskeleston organization
| العنوان: | Transgelin is a TGFβ-inducible gene that regulates osteoblastic and adipogenic differentiation of human skeletal stem cells through actin cytoskeleston organization |
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| المؤلفون: | Musaed Alfayez, Rimi Hamam, Mona Elsafadi, Nehad M. Alajez, Muthurangan Manikandan, Abdullah Aldahmash, Amer Mahmood, Moustapha Kassem, Raed Abu Dawud |
| المصدر: | Cell Death & Disease Elsafadi, E, Manikandan, M, Dawud, R A, Alajez, N M, Hamam, R, Alfayez, M, Kassem, M, Aldahmash, A & Mahmood, A 2016, ' Transgelin is a TGF β-inducible gene that regulates osteoblastic and adipogenic differentiation of human skeletal stem cells through actin cytoskeleston organization ', Cell Death & Disease, vol. 7, no. 8, e2321, pp. e2321 . https://doi.org/10.1038/cddis.2016.196Test |
| بيانات النشر: | Nature Publishing Group, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Cancer Research, Cytoskeleton organization, Cellular differentiation, Muscle Proteins, 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit, RNA, Small Interfering/metabolism, 0302 clinical medicine, Cell Movement, Transforming Growth Factor beta, Adipocytes, RNA, Small Interfering, Actin Cytoskeleton/metabolism, Muscle Proteins/genetics, Adipogenesis, Adipocytes/cytology, Microfilament Proteins, Cell migration, Cell biology, Actin Cytoskeleton, 030220 oncology & carcinogenesis, Original Article, Stem cell, Adipogenesis/genetics, Transforming Growth Factor beta/metabolism, Signal Transduction, Stromal cell, Immunology, Foreskin, Biology, 03 medical and health sciences, Cellular and Molecular Neuroscience, Cell Proliferation/genetics, Microfilament Proteins/genetics, Cell Movement/genetics, Humans, Progenitor cell, Muscle, Skeletal, Cell Proliferation, Cell Nucleus, Osteoblasts, Cell growth, Mesenchymal Stem Cells/cytology, Gene Expression Profiling, Osteoblasts/cytology, Mesenchymal Stem Cells, Cell Biology, Fibroblasts, Actin cytoskeleton, equipment and supplies, 030104 developmental biology, Fibroblasts/cytology, Muscle, Skeletal/cytology, Foreskin/cytology, Cell Nucleus/metabolism, Cancer research, Signal Transduction/genetics |
| الوصف: | Regenerative medicine is a novel approach for treating conditions in which enhanced bone regeneration is required. We identified transgelin (TAGLN), a transforming growth factor beta (TGFβ)-inducible gene, as an upregulated gene during in vitro osteoblastic and adipocytic differentiation of human bone marrow-derived stromal (skeletal) stem cells (hMSC). siRNA-mediated gene silencing of TAGLN impaired lineage differentiation into osteoblasts and adipocytes but enhanced cell proliferation. Additional functional studies revealed that TAGLN deficiency impaired hMSC cell motility and in vitro transwell cell migration. On the other hand, TAGLN overexpression reduced hMSC cell proliferation, but enhanced cell migration, osteoblastic and adipocytic differentiation, and in vivo bone formation. In addition, deficiency or overexpression of TAGLN in hMSC was associated with significant changes in cellular and nuclear morphology and cytoplasmic organelle composition as demonstrated by high content imaging and transmission electron microscopy that revealed pronounced alterations in the distribution of the actin filament and changes in cytoskeletal organization. Molecular signature of TAGLN-deficient hMSC showed that several genes and genetic pathways associated with cell differentiation, including regulation of actin cytoskeleton and focal adhesion pathways, were downregulated. Our data demonstrate that TAGLN has a role in generating committed progenitor cells from undifferentiated hMSC by regulating cytoskeleton organization. Targeting TAGLN is a plausible approach to enrich for committed hMSC cells needed for regenerative medicine application. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2041-4889 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7291ae5b87745bbb106c8f83602d32eTest http://europepmc.org/articles/PMC5108308Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c7291ae5b87745bbb106c8f83602d32e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20414889 |
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