التفاصيل البيبلوغرافية
| العنوان: |
Selective killing of CD8+ cells with a `memory' phenotype (CD62Llo) by the N-acetyl-D-galactosamine-specific lectin from Viscum album L. |
| المؤلفون: |
Büssing, Arndt, Stein, Gerburg M, Pfüller, Uwe |
| المصدر: |
Cell Death & Differentiation. Mar1998, Vol. 5 Issue 3, p231. 10p. |
| مصطلحات موضوعية: |
*PROTEINS, *EUROPEAN mistletoe, *APOPTOSIS, *LYMPHOCYTES |
| مستخلص: |
As reported previously by our group, among the toxic proteins from Viscum album L. only the mistletoe lectins (MLs) induce the apoptotic killing pathway in human lymphocytes. Although one may expect a homogenous distribution of carbohydrate domains on cell surface receptors for the carbohydrate binding B chains of the toxic protein, the sensitivity of cells to these B chains obviously differ. Here we report a selective killing of CD8[sup +] CD62L[sup lo] cells from healthy individuals by the galNAcspecific ML III (and RCA[sub 60], which binds to gal and galNAc), while the gal-specific ML I was less effective. This selective killing is not sufficiently explained by protein synthesis inhibition alone, since this subset was not affected by other ribosome inhibiting proteins such as the lectin from Ricinus communis (RCA[sub 120]), lectin from Abrus precatorus (APA), abrin A, and inhibitors of RNA, DNA and/or protein synthesis such as actinomycin D, mitomycin C, and cycloheximide. We conclude that CD8[sup +] cells with 'memory' phenotype (CD62L[sup lo]) are more sensitive to the ML Illmediated killing than their CD8[sup +] CD62L[sup hi] counterparts, CD4[sup +] T cells, and CD19[sup +] B cells. These cells probably express a distinct receptor with galNAc domains that is missing or not active on CD8[sup +] cells with a 'naive' phenotype. [ABSTRACT FROM AUTHOR] |
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