B-Myb promotes S-phase independently of its sequence-specific DNA binding activity and interacts with polymerase delta-interacting protein 1 (Pdip1)
| العنوان: | B-Myb promotes S-phase independently of its sequence-specific DNA binding activity and interacts with polymerase delta-interacting protein 1 (Pdip1) |
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| المؤلفون: | Thore Schmedt, Karl-Heinz Klempnauer, Heiko Hoffmann, Eugen Werwein, Jeffrey D. Singer, Clemens Usadel, Nora Obermann |
| المصدر: | Cell Cycle. 11:4047-4058 |
| بيانات النشر: | Informa UK Limited, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | DNA Replication, animal structures, HMG-box, Cell Cycle Proteins, Biology, Transfection, DNA polymerase delta, Cell Line, RFC2, Replication factor C, Transcription (biology), Report, Proliferating Cell Nuclear Antigen, Sequence-specific DNA binding, Animals, Humans, MYB, RNA, Small Interfering, Molecular Biology, Transcription factor, fungi, Nuclear Proteins, DNA, Hep G2 Cells, Cell Biology, Molecular biology, Cell biology, HEK293 Cells, Mutation, S Phase Cell Cycle Checkpoints, Trans-Activators, RNA Interference, Chickens, Protein Binding, Developmental Biology |
| الوصف: | B-Myb is a highly conserved member of the Myb transcription factor family, which plays an essential role in cell cycle progression by regulating the transcription of genes at the G 2/M-phase boundary. The role of B-Myb in other parts of the cell cycle is less well-understood. By employing siRNA-mediated silencing of B-Myb expression, we found that B-Myb is required for efficient entry into S-phase. Surprisingly, a B-Myb mutant that lacks sequence-specific DNA-binding activity and is unable to activate transcription of B-Myb target genes is able to rescue the S-phase defect observed after B-Myb knockdown. Moreover, we have identified polymerase delta-interacting protein 1 (Pdip1), a BTB domain protein known to bind to the DNA replication and repair factor PCNA as a novel B-Myb interaction partner. We have shown that Pdip1 is able to interact with B-Myb and PCNA simultaneously. In addition, we found that a fraction of endogenous B-Myb can be co-precipitated via PCNA, suggesting that B-Myb might be involved in processes related to DNA replication or repair. Taken together, our work suggests a novel role for B-Myb in S-phase that appears to be independent of its sequence-specific DNA-binding activity and its ability to stimulate the expression of bona fide B-Myb target genes. |
| تدمد: | 1551-4005 1538-4101 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be5a341523da4722283594e49e7a0a1fTest https://doi.org/10.4161/cc.22386Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....be5a341523da4722283594e49e7a0a1f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15514005 15384101 |
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