Upregulation of circ‐FBL promotes myogenic proliferation in myasthenia gravis by regulation of miR‐133/PAX7
| العنوان: | Upregulation of circ‐FBL promotes myogenic proliferation in myasthenia gravis by regulation of miR‐133/PAX7 |
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| المؤلفون: | Xiaoyin Lai, Zhuajin Bi, Lu Wang, Rongguo Hu, Longxuan Li, Xuelian Yang, Mingming Jin, Bitao Bu |
| المصدر: | Cell Biology International. 45:2287-2293 |
| بيانات النشر: | Wiley, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Myoblast proliferation, Neuromuscular transmission, Apoptosis, Biology, Muscle Development, Proinflammatory cytokine, Mice, Downregulation and upregulation, Cell Movement, Circular RNA, Cell Line, Tumor, Myasthenia Gravis, microRNA, medicine, Animals, Humans, RNA, Messenger, Cell Proliferation, Messenger RNA, PAX7 Transcription Factor, Cell Differentiation, RNA, Circular, Cell Biology, General Medicine, medicine.disease, Myasthenia gravis, Mice, Inbred C57BL, MicroRNAs, Cancer research |
| الوصف: | Myasthenia gravis (MG) is a disease involving neuromuscular transmission that causes fatigue of skeletal muscles and fluctuating weakness. It has been shown that impairment of myogenic differentiation and myofiber maturation may be the underlying cause of MG. In this study, we detected the abnormal expression of circular RNA (circRNA) using next-generation sequencing in patients with MG. We then investigated the regulatory mechanism and the relationship among circRNA, microRNA, and messenger RNA using quantitative reverse-transcription polymerase chain reaction, bioinformatics analysis, and luciferase report analysis. The expression of inflammatory cytokines and regulatory T lymphocytes was shown to be increased. Circ-FBL was significantly increased in MG patients. Bioinformatics and luciferase report analyses confirmed that miR-133 and PAX7 were the downstream targets of circ-FBL. Overexpression of circ-FBL promoted myoblast proliferation by regulation of miR-133/PAX7. Taken together, our study showed that upregulation of circ-FBL promoted myogenic proliferation in patients with MG by regulating miR-133/PAX7. |
| تدمد: | 1095-8355 1065-6995 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f055415ae5df6857be4d60d8b8782dfTest https://doi.org/10.1002/cbin.11676Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4f055415ae5df6857be4d60d8b8782df |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10958355 10656995 |
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