دورية
MYCOBACTERIAL GROWTH IN HUMAN MACROPHAGES: VARIATION ACCORDING TO DONOR, INOCULUM AND BACTERIAL STRAIN
| العنوان: | MYCOBACTERIAL GROWTH IN HUMAN MACROPHAGES: VARIATION ACCORDING TO DONOR, INOCULUM AND BACTERIAL STRAIN |
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| المؤلفون: | Hoal-van Helden, E.G., Hon, D., Lewis, L.-A., Beyers, N., van Helden, P.D. |
| المصدر: | Cell Biology International; January 2001, Vol. 25 Issue: 1 p71-81, 11p |
| مستخلص: | The microbicidal capacity of the macrophage is frequently evaded by mycobacteria, leading to tuberculosis (TB). We investigated a number of parameters affecting the rate of growth of mycobacteria in human monocyte-derived macrophages (MDM). The results show a great deal of variation in the growth of both Mycobacterium bovisBCG and M. tuberculosisH37Rv, using a large number of human macrophage donors, (132 and 40, respectively), but no correlation was seen with the TB status of the MDM donor. Clumping of the mycobacteria resulted in more vigorous growth in MDM, suggesting that inoculum size could affect disease progression. The growth rates of 17 clinical isolates of M. tuberculosiswere measured in macrophages derived from three donors and no consistent or marked differences between isolates were observed over the 5-day period of growth measurement. However, all 17 clinical strains grew consistently faster than H37Rv in the same experiments. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 10656995 10958355 |
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| DOI: | 10.1006/cbir.2000.0679 |