KIF5B modulates central spindle organization in late-stage cytokinesis in chondrocytes
| العنوان: | KIF5B modulates central spindle organization in late-stage cytokinesis in chondrocytes |
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| المؤلفون: | Huiyan Gan, Daniel W. Chan, W Xue, Jian-Dong Huang, Guixia Zhu, Kathryn S.E. Cheah, Ya Gao |
| المصدر: | Cell & Bioscience Cell & Bioscience, Vol 9, Iss 1, Pp 1-16 (2019) |
| بيانات النشر: | BioMed Central, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, lcsh:Biotechnology, General Biochemistry, Genetics and Molecular Biology, Chondrocyte, Motor protein, lcsh:Biochemistry, 03 medical and health sciences, 0302 clinical medicine, Chondrocytes, Microtubule, lcsh:TP248.13-248.65, Kinesin-1, medicine, KIF3A, lcsh:QD415-436, Central spindle, lcsh:QH301-705.5, Cytokinesis, Chemistry, Research, Chondrogenesis, Cell biology, 030104 developmental biology, medicine.anatomical_structure, lcsh:Biology (General), 030220 oncology & carcinogenesis, Kinesin |
| الوصف: | Background The growth plate is a special region of the cartilage that drives longitudinal growth of long bones. Proliferating chondrocytes in the growth plate, arranged in columns, divide perpendicular to the long axis of the growth plate then intercalate to re-align with parental columns. Which molecular partners maintain growth plate columnar structures and chondrocyte cytokinesis has not been fully revealed. It is reported that kinesin family member 3A (KIF3A), a subunit of kinesin-2, plays an important role in maintaining columnar organization in growth plates via controlling primary cilia formation and cell proliferation. Result Here we identify kinesin family member 5B (KIF5B), the heavy chain of kinesin-1, a ubiquitously expressed motor protein for anterograde intracellular transport along the microtubule network, as a key modulator of cytokinesis in chondrocytes via maintenance of central spindle organization. We show that KIF5B is concentrated in the central spindle during cytokinesis in both primary chondrocytes and chondrogenic ATDC5 cells. Conclusion The failure of cytokinesis in KIF5B null chondrocytes leads to incomplete cell rotation, disrupting proliferation and differentiation, and results in a disorganized growth plate. |
| اللغة: | English |
| تدمد: | 2045-3701 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::08c39e154691d9f3d552bdf7573e404dTest http://europepmc.org/articles/PMC6794761Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....08c39e154691d9f3d552bdf7573e404d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20453701 |
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