المؤلفون: Stephen M. Hedrick

المصدر: Cell. 70(2)

مصطلحات موضوعية: biology, Antigen presentation, Histocompatibility Antigens Class I, Histocompatibility Antigens Class II, Bacterial Infections, Major histocompatibility complex, General Biochemistry, Genetics and Molecular Biology, Major Histocompatibility Complex, Minor Histocompatibility Antigens, Immunology, Minor histocompatibility antigen, biology.protein, Animals, Function (biology)

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ac6a6b7d49ac6682c47462933559f82Test
https://pubmed.ncbi.nlm.nih.gov/1322246Test

المؤلفون: Michael N. Margolies, Jonathan Kaye, Ralph T. Kubo, Meredith Mudgett-Hunter, Richard I. Near, Michael L.B. Becker, Stephen M. Hedrick

المصدر: Cell. 58:911-921

مصطلحات موضوعية: Antigens, Differentiation, T-Lymphocyte, Digoxin, CD3 Complex, Macromolecular Substances, Receptors, Antigen, T-Cell, alpha-beta, T-Lymphocytes, CD3, T cell, Receptors, Antigen, T-Cell, Mice, Transgenic, General Biochemistry, Genetics and Molecular Biology, Recombination-activating gene, Mice, Structure-Activity Relationship, medicine, Animals, Receptor, Alleles, Genes, Immunoglobulin, biology, T-cell receptor, Gene rearrangement, Molecular biology, Recombinant Proteins, Allelic exclusion, medicine.anatomical_structure, biology.protein, Antibody, Immunoglobulin Heavy Chains

الوصف: We have constructed a hybrid immunoglobulin (VDJH)-T cell receptor (C alpha) gene using the VDJH exon from a digoxin-specific antibody. This gene was used to make a line of transgenic mice. The hybrid VDJH-C alpha protein is expressed on a subset of T cells in these mice, and we have shown that it forms part of a functional TCR complex by the criteria of coprecipitation and comodulation of CD3 and TCR beta chain components and T cell activation with anti-idiotypic antibodies or digoxin. Furthermore, in cells expressing the hybrid protein, there is allelic exclusion of endogenous TCR alpha genes. We discuss the implications for the comparative structure of T cell receptors and immunoglobulins.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a53c072f917af64648201363d213c5bTest
https://doi.org/10.1016/0092-8674Test(89)90943-4

المؤلفون: Isaac Engel, Stephen M. Hedrick

المصدر: Cell. 54:473-484

مصطلحات موضوعية: Cytochrome, T cell, Molecular Sequence Data, Receptors, Antigen, T-Cell, Cytochrome c Group, chemical and pharmacologic phenomena, Transfection, medicine.disease_cause, General Biochemistry, Genetics and Molecular Biology, Epitope, Epitopes, Mice, medicine, Animals, Amino Acid Sequence, Peptide sequence, chemistry.chemical_classification, Mutation, biology, Cytochrome c, T-cell receptor, Nucleic Acid Hybridization, hemic and immune systems, Molecular biology, Amino acid, medicine.anatomical_structure, chemistry, biology.protein

الوصف: In order to assess the importance of a conserved amino acid in the VDJ junctional region of the beta chain of T cell receptors (TCRs) specific for pigeon cytochrome c, we generated T cell transfectant clones that express either a TCR identical to that of the cytochrome c-specific clone D6 or a mutated form of the D6 TCR, in which the conserved residue was replaced by one of two other amino acids. We have found that one substitution alters antigen fine specificity, while the other substitution abolishes all detectable cytochrome c response. On the basis of these findings, we propose that this conserved amino acid is a key residue in determining the antigen specificity of the D6 TCR.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3430e37629a6e8a969b721378af916f4Test
https://doi.org/10.1016/0092-8674Test(88)90068-2

المؤلفون: Jack D. Bui, Sébastien Calbo, Stephen M. Hedrick, Kristine Hayden-Martinez, Phyllis Gardner, Lawrence P. Kane

المصدر: Cell. (4):457-467

مصطلحات موضوعية: Genetically modified mouse, medicine.medical_specialty, Calmodulin, Lymphoid Tissue, T-Lymphocytes, Cellular differentiation, T cell, Mice, Transgenic, Thymus Gland, Lymphocyte Activation, General Biochemistry, Genetics and Molecular Biology, Immunophenotyping, Interferon-gamma, Mice, Ca2+/calmodulin-dependent protein kinase, Internal medicine, medicine, Animals, Humans, Cytotoxic T cell, Transgenes, IL-2 receptor, biology, Kinase, Biochemistry, Genetics and Molecular Biology(all), Cell biology, Isoenzymes, medicine.anatomical_structure, Endocrinology, Calcium-Calmodulin-Dependent Protein Kinases, biology.protein, Interleukin-4, Calcium-Calmodulin-Dependent Protein Kinase Type 2, Immunologic Memory, Cell Division

الوصف: In order to study the role of calcium/calmodulin kinase II (CaMKII) in T cells, we generated transgenic mice expressing CaMKIIγB* (T287D), a partially calciumindependent mutant of CaMKIIγB. In these mice, the size of the thymus was increased 1.5- to 2-fold, at least in part due to an increase in the lifespan of double-positive (DP) thymocytes. More importantly, there was an increase in the number of T cells in the secondary lymphoid organs that had acquired an antigen-dependent memory phenotype. These T cells were bonafide memory cells as assessed by a variety of criteria. In addition, T cells from wild-type mice acquired calcium-independent CaMKII activity after several rounds of antigen-stimulated division. We propose that CaMKII controls a distinct process of activation-induced cellular differentiation.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1888c5d34b5ffa8a8ed49048fccca2beTest

المؤلفون: Stephen M. Hedrick, Ellen Heber-Katz, Lawrence E. Samelson, Toby T. Hecht, Dan L. Longo, Ronald H. Schwartz, Louis A. Matis

المصدر: Cell. 30(1)

مصطلحات موضوعية: T cell, T-Lymphocytes, Genes, MHC Class II, Receptors, Antigen, T-Cell, chemical and pharmacologic phenomena, Cytochrome c Group, Biology, Cross Reactions, Major histocompatibility complex, General Biochemistry, Genetics and Molecular Biology, Epitopes, Mice, Antigen, medicine, Animals, Antigen-presenting cell, Columbidae, Immune response gene, Hybridomas, Cytochrome c, Macrophages, T-cell receptor, Histocompatibility Antigens Class II, MHC restriction, Molecular biology, medicine.anatomical_structure, biology.protein, Interleukin-2

الوصف: The activation of proliferative T lymphocytes normally involves the simultaneous recognition of a particular foreign antigen and a particular Ia molecule on the surface of antigen-presenting cells, the phenomenon of major histocompatibility complex (MHC) restriction. An analysis of T cell clones specific for pigeon cytochrome c, from B10.A and B10.S(9R) strains of mice, revealed the unusual finding that several of the clones could respond to antigen in association with la molecules from either strain. Using these cross-reactive clones, we performed experiments which demonstrated that both the la molecule and the T cell receptor contribute to the specificity of antigen recognition; however, MHC-linked low responsiveness to tuna cytochrome c (an immune response gene defect) could not be attributed solely to the efficacy with which the la molecules associated with the antigen. These results imply that antigen and Ia molecules are not recognized independently, but must interact at least during the process of T cell activation.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3887deb97935dbf8af87a2c606f8b77dTest
https://pubmed.ncbi.nlm.nih.gov/6181895Test

المؤلفون: Simona B. Sorger, Pamela J. Fink, Stephen M. Hedrick, David L. McElligott, Louis A. Matis

المصدر: Cell. 51(1)

مصطلحات موضوعية: Male, Cellular immunity, Isoantigens, T cell, DNA, Recombinant, Receptors, Antigen, T-Cell, chemical and pharmacologic phenomena, Cytochrome c Group, Biology, Moths, Major histocompatibility complex, Lymphocyte Activation, General Biochemistry, Genetics and Molecular Biology, Major Histocompatibility Complex, Epitopes, Mice, Antigen, Cell surface receptor, medicine, Animals, Allorecognition, Columbidae, Genetics, Genes, Immunoglobulin, T-cell receptor, Histocompatibility Antigens Class II, Nucleic Acid Hybridization, T lymphocyte, DNA, T-Lymphocytes, Helper-Inducer, Molecular biology, Clone Cells, medicine.anatomical_structure, biology.protein, Female

الوصف: We have studied the relationship between major histocompatibility complex (MHC)-restricted antigen recognition and alloreactivity by examining T cell receptor (TCR) alpha and beta gene expression in cytochrome c-specific, Ek alpha:Ek beta (Ek)-restricted helper T cell clones derived from B10.A mice. The clones could be segregated on the basis of four distinct alloreactivity patterns. Clones cross-reactive for three different allogeneic la molecules (As alpha:As beta [As], Ab alpha:Ab beta [Ab], Ek alpha: Eb beta [Eb]) expressed the same V alpha and V beta gene segments, generating the distinct alloreactive specificities via unique V alpha-J alpha and V beta-D beta-J beta joining events. Ek alpha:Es beta (Es)-alloreactive B10.A clones expressed the same V alpha, J alpha, and V beta segments as an Es-restricted, Ek-alloreactive, cytochrome c-specific, H-2-congenic B10.S(9R) clone. This homology between TCRs mediating allorecognition of la molecules and recognition of the same la molecules as restriction elements associated with nominal antigen suggests that MHC-restricted recognition and allorecognition represent differences in the affinity of the TCR-MHC molecule interaction.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52208f74fa3d0c33873be5201189f88aTest
https://pubmed.ncbi.nlm.nih.gov/2443252Test