التفاصيل البيبلوغرافية
| العنوان: |
Differences in the Active Endometrial Microbiota across Body Weight and Cancer in Humans and Mice. |
| المؤلفون: |
Kaakoush, Nadeem O.1 (AUTHOR) n.kaakoush@unsw.edu.au, Olzomer, Ellen M.2 (AUTHOR) e.olzomer@unsw.edu.au, Kosasih, Melidya3 (AUTHOR) melidya.kosasih@health.wa.gov.au, Martin, Amy R.3,4 (AUTHOR) amyrose.martin@health.nsw.gov.au, Fargah, Farokh5 (AUTHOR) farokh.fargah@laverty.com.au, Lambie, Neil6 (AUTHOR) neil.lambie@cdhb.health.nz, Susic, Daniella3 (AUTHOR) d.susic@unsw.edu.au, Hoehn, Kyle L.2 (AUTHOR) k.hoehn@unsw.edu.au, Farrell, Rhonda7,8 (AUTHOR) rhonda.farrell@lh.org.au, Byrne, Frances L.2 (AUTHOR) frances.byrne@unsw.edu.au |
| المصدر: |
Cancers. May2022, Vol. 14 Issue 9, pN.PAG-N.PAG. 18p. |
| مصطلحات موضوعية: |
*OBESITY complications, *BODY weight, *SEQUENCE analysis, *ANIMAL experimentation, *LEANNESS, *HUMAN microbiota, *ENDOMETRIAL tumors, *DISEASE prevalence, *LACTOBACILLUS, *BODY mass index, *ENDOMETRIUM, *MICE, *BACTERIA, *DISEASE risk factors |
| مستخلص: |
Simple Summary: Of all cancers, endometrial cancer has the greatest association with obesity. Obesity causes dysbiosis of intestinal microbiota, but little is known about whether obesity is associated with dysbiosis of the female genital tract. Therefore, the aim of this study was to determine whether obesity and cancer were associated with altered microbiota profiles in the endometrium. 16S rRNA transcript amplicon sequencing (which captures actively replicating bacteria) of endometrial tissues showed that obesity and cancer were associated with the prevalence of microbial community types in the human endometrium. However, obesity was not associated with microbial community types in the mouse endometrium. The presence of endometrial cancer (but not obesity) was associated with decreased abundance of the Lactobacillus genus in the human endometrium. In mice, an enrichment of Lactobacillus was associated with lower prevalence of disease (normal uterine histology). These results suggest that obesity and cancer may influence microbiota community types in the endometrium (at least in humans) and Lactobacillus may be protective in the endometrium. This study therefore supports further research into the role of microbiota in endometrial cancer development. Obesity is a risk factor for endometrial cancer. The aim of this study was to determine whether actively replicating microbiota in the endometrium differ between obese vs. lean and cancer vs. benign states. We performed 16S rRNA amplicon sequencing on endometrial tissues from lean and obese women with and without endometrial cancer, and lean and obese mice. Results displayed human endometrial microbiota clustered into three community types (R = 0.363, p = 0.001). Lactobacillus was dominant in community type 1 (C1) while community type 2 (C2) had high levels of Proteobacteria and more cancer samples when compared to C1 (p = 0.007) and C3 (p = 0.0002). A significant increase in the prevalence of the C2 community type was observed across body mass index and cancer (χ2 = 14.24, p = 0.0002). The relative abundance of Lactobacillus was lower in cancer samples (p = 0.0043), and an OTU with 100% similarity to Lactobacillus iners was enriched in control samples (p = 0.0029). Mouse endometrial microbiota also clustered into three community types (R = 0.419, p = 0.001) which were not influenced by obesity. In conclusion, obesity and cancer are associated with community type prevalence in the human endometrium, and Lactobacillus abundance is associated with normal uterine histologies in humans and mice. [ABSTRACT FROM AUTHOR] |
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