Endogenous Two-Photon Fluorescence Imaging Elucidates Metabolic Changes Related to Enhanced Glycolysis and Glutamine Consumption in Precancerous Epithelial Tissues
| العنوان: | Endogenous Two-Photon Fluorescence Imaging Elucidates Metabolic Changes Related to Enhanced Glycolysis and Glutamine Consumption in Precancerous Epithelial Tissues |
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| المؤلفون: | Kyongbum Lee, Carlo Amadeo Alonzo, Dimitra Pouli, Joanna Xylas, Antonio Varone, Kyle P. Quinn, Gautham V. Sridharan, Irene Georgakoudi, Karl Münger, Margaret E. McLaughlin-Drubin |
| المصدر: | Cancer Research. 74:3067-3075 |
| بيانات النشر: | American Association for Cancer Research (AACR), 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Diagnostic Imaging, Cancer Research, Bioenergetics, Glutamine, Endogeny, Oxidative phosphorylation, Biology, Fluorescence, Oxidative Phosphorylation, Article, medicine, Humans, Glycolysis, Cells, Cultured, Tissue Engineering, Cancer, Epithelial Cells, Two photon fluorescence, medicine.disease, Cell biology, Metabolic pathway, Microscopy, Fluorescence, Multiphoton, Oncology, Biochemistry, sense organs, Energy Metabolism, Oxidation-Reduction, Precancerous Conditions, Biomarkers, Metabolic Networks and Pathways |
| الوصف: | Alterations in the balance between different metabolic pathways used to meet cellular bioenergetic and biosynthetic demands are considered hallmarks of cancer. Optical imaging relying on endogenous fluorescence has been used as a noninvasive approach to assess tissue metabolic changes during cancer development. However, quantitative correlations of optical assessments with variations in the concentration of relevant metabolites or in the specific metabolic pathways that are involved have been lacking. In this study, we use high-resolution, depth-resolved imaging, relying entirely on endogenous two-photon excited fluorescence in combination with invasive biochemical assays and mass spectrometry to demonstrate the sensitivity and quantitative nature of optical redox ratio tissue assessments. We identify significant differences in the optical redox ratio of live, engineered normal and precancerous squamous epithelial tissues. We establish that while decreases in the optical redox ratio are associated with enhanced levels of glycolysis relative to oxidative phosphorylation, increases in glutamine consumption to support energy production are associated with increased optical redox ratio values. Such mechanistic insights in the origins of optical metabolic assessments are critical for exploiting fully the potential of such noninvasive approaches to monitor and understand important metabolic changes that occur in live tissues at the onset of cancer or in response to treatment. Cancer Res; 74(11); 3067–75. ©2014 AACR. |
| تدمد: | 1538-7445 0008-5472 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ed07319834dc092c9fd864be6b0caccTest https://doi.org/10.1158/0008-5472.can-13-2713Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4ed07319834dc092c9fd864be6b0cacc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15387445 00085472 |
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