Dysbiosis signatures of the microbial profile in tissue from bladder cancer
| العنوان: | Dysbiosis signatures of the microbial profile in tissue from bladder cancer |
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| المؤلفون: | Liu Fei, Yinghao Sun, Lu Xin, Qiao Xiong, Yongping Xue, Haoyuan Tan, Chuanliang Xu, Weidong Xu, Anwei Liu, Shuxiong Zeng, Jinshan Xu, He Xing, Zhensheng Zhang |
| المصدر: | Cancer Medicine, Vol 8, Iss 16, Pp 6904-6914 (2019) Cancer Medicine |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Cancer Research, Urinary Bladder, lcsh:RC254-282, Microbiology, 03 medical and health sciences, 0302 clinical medicine, RNA, Ribosomal, 16S, Parenchyma, microbiota, medicine, Prevotella, Humans, Radiology, Nuclear Medicine and imaging, Microbiome, 16s rRNA, Original Research, Aged, urinary tract, Mucous Membrane, Bladder cancer, biology, Clinical Cancer Research, Cancer, Middle Aged, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, medicine.disease, biology.organism_classification, 030104 developmental biology, Urinary Bladder Neoplasms, Oncology, 030220 oncology & carcinogenesis, Dysbiosis, bladder cancer, Female, Proteobacteria, Ruminococcaceae |
| الوصف: | Background To examine the microbial profiles in parenchyma tissues in bladder cancer. Methods Tissue samples of cancerous bladder mucosa were collected from patients diagnosed with bladder cancer (22 carcinoma tissues and 12 adjacent normal tissues). The V3‐V4 region of the bacterial 16S rRNA gene was PCR amplified, followed by sequencing on an Illumina MiSeq platform. Bioinformatics analysis for microbial classification and functional assessment was performed to assess bladder microbiome diversity and variations. Results The predominant phylum in both tissues was Proteobacteria. The cancerous tissues exhibited lower species richness and diversity. Beta diversity significantly differed between the cancerous and normal tissues. Lower relative abundances of the microbial genera Lactobacillus, Prevotella_9, as well as Ruminococcaceae were observed, whereas those of Cupriavidus spp., an unknown genus of family Brucellaceae, and Acinetobacter, Anoxybacillus, Escherichia‐Shigella, Geobacillus, Pelomonas, Ralstonia, and Sphingomonas were higher in the cancerous tissues. These findings indicate that these genera may be potentially utilized as biomarkers for bladder cancer. PICRUSt analysis revealed that several pathways involved in the metabolism of harmful chemical compounds were enriched in the cancer tissues, thereby providing evidence that environmental factors are strongly associated with bladder cancer etiology. Conclusion This is the first study that has described and analyzed the dysbiotic motifs of urinary microbiota in the parenchymatous tissues of bladder cancer via 16S rRNA gene sequencing. Our results suggest that changes in the bladder microbiome may serve as biomarkers for bladder cancer, possibly assisting in disease screening and monitoring. This is the first study describing and analyzing the dysbiosis signatures of urinary microbiota within the parenchyma in bladder cancer using a 16S rRNA gene sequencing method. The study reveals previously undescribed bacterial diversity present in human bladder. The bladder microbiome changes could be a biomarker of bladder cancer that could be used to help in screening for the disease. Analysis of the inferred bladder cancer metagenome identified the strongest association with the harmful chemical products that may be metabolized. |
| تدمد: | 2045-7634 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bbd6331f0efcb72046251eaf84bb6c32Test https://doi.org/10.1002/cam4.2419Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bbd6331f0efcb72046251eaf84bb6c32 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20457634 |
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