Tumor quantification of several fluoropyrimidines resistance gene expression with a unique quantitative RT-PCR method. Implications for pretherapeutic determination of tumor resistance phenotype
| العنوان: | Tumor quantification of several fluoropyrimidines resistance gene expression with a unique quantitative RT-PCR method. Implications for pretherapeutic determination of tumor resistance phenotype |
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| المؤلفون: | Erick Gamelin, Gérard Lorimier, Alain Morel, Véronique Verriele, Laurence Preisser, Maud Barbado, Michèle Boisdron-Celle |
| المساهمون: | Cytokines : structure, signalisation et prolifération tumorale, Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire d'Anatomopathologie, Centre régional de lutte contre le cancer, Departement de Chirurgie Oncologique, CRLCC Paul Papin, Ligue contre le Cancer du Maine et Loire, Universite d'Angers, predoctoral fellowship from the Conseil Regional des Pays de Loire, posdoctoral fellowship from La Ligue Nationale Contre le Cancer, Collaboration, Canepari, Marco |
| المصدر: | Cancer Letters Cancer Letters, Elsevier, 2006, 242 (2), pp.168-79. ⟨10.1016/j.canlet.2005.11.008⟩ |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | MESH: Carcinoma, Cancer Research, Colorectal cancer, Biopsy, Thymidylate synthase, MESH: Biopsy, 0302 clinical medicine, MESH: Reverse Transcriptase Polymerase Chain Reaction, Dihydrofolate reductase, Intestinal Mucosa, Oligonucleotide Array Sequence Analysis, 0303 health sciences, MESH: Antimetabolites, Antineoplastic, biology, Reverse Transcriptase Polymerase Chain Reaction, MESH: Gene Expression Regulation, Neoplastic, Phenotype, MESH: Drug Resistance, Neoplasm, 3. Good health, Gene Expression Regulation, Neoplastic, Real-time polymerase chain reaction, Oncology, 030220 oncology & carcinogenesis, MESH: Intestinal Mucosa, Fluorouracil, Colorectal Neoplasms, MESH: DNA Primers, Antimetabolites, Antineoplastic, DNA, Complementary, [SDV.CAN]Life Sciences [q-bio]/Cancer, MESH: Phenotype, 03 medical and health sciences, [SDV.CAN] Life Sciences [q-bio]/Cancer, medicine, Dihydropyrimidine dehydrogenase, Humans, 030304 developmental biology, DNA Primers, MESH: Humans, Carcinoma, MESH: DNA, Complementary, medicine.disease, Molecular biology, Pyrimidines, Thymidine kinase, Drug Resistance, Neoplasm, MESH: Pyrimidines, Cancer cell, MESH: Oligonucleotide Array Sequence Analysis, biology.protein, MESH: Fluorouracil, MESH: Colorectal Neoplasms |
| الوصف: | International audience; Pretherapeutic determination of tumor resistance to chemotherapy is a main challenge, hindered by the low number of mechanisms characterized at the same time, the small size of the clinical specimens and the heterogeneity of the techniques or the lack of true quantification. The aim of the present study was to determine in real time quantitative RT-PCR, tumor cell expression of several transcripts involved in cancer cell resistance with a unique cDNA sample from a tumor biopsy. The technique had to be suitable in clinical practice for determination of several factors involved in resistance to a given drug family, for example, fluoropyrimidines resistance factors: thymidylate synthase (TS), dihydropyrimidine dehydrogenase (DPD), thymidine kinase (TK), dihydrofolate reductase (DHFR), folylpolyglutamate synthetase (FPGS). A frame-shifted artificial construct was designed specifically to work within the same conditions. We validated our technique by quantifying expressions of these 5 genes starting from tissue samples of colorectal carcinoma and the surrounding normal mucosa of 33 different patients. That real time quantitative RT-PCR technique using the frame-shifted artificial construct as a standard provided a real comparison and quantification of different resistance factors. Tumor resistance phenotype determination based on that approach will be investigated in a control study. |
| وصف الملف: | application/pdf |
| تدمد: | 0304-3835 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57bb6de76bc6e8a63d9cca9832baa7e1Test https://pubmed.ncbi.nlm.nih.gov/16387426Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....57bb6de76bc6e8a63d9cca9832baa7e1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 03043835 |
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