Proteins in aqueous extracts of breast muscle, leg muscle, cardiac muscle, liver, kidney, and spleen of adult cockerels were compared by zone electrophoresis in starch gel in sodium cacodylate or sodium citrate – phosphate systems. The proteins and enzymes were located in the starch gel by a variety of histochemical techniques. A partial characterization of the tissue esterases was carried out with various substrates and specific inhibitors. The 18 bands of esterases detected in tissues were aliesterases of two different types. Tissue specific enzymic patterns were obtained for esterases, alkaline phosphatase, acid phosphatase, lactate dehydrogenase, malate dehydrogenase, and isocitrate dehydrogenase. Glutamate dehydrogenase and glucose-6-phosphate dehydrogenase activity were detected only in the liver and kidney.
