Establishment and Characterization of a Culture System for Enzymatically Released Rat Dental Pulp Cells
| العنوان: | Establishment and Characterization of a Culture System for Enzymatically Released Rat Dental Pulp Cells |
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| المؤلفون: | I. Katayama, T. Matsuoka, Hiroshi Kadokura, Tadashi Katayama, Fujieda K, Yoshifumi Tajima, Satoshi Yokose |
| المصدر: | Calcified Tissue International. 66:139-144 |
| بيانات النشر: | Springer Science and Business Media LLC, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Time Factors, Transcription, Genetic, Endocrinology, Diabetes and Metabolism, Osteocalcin, Cell Culture Techniques, Odontoblast differentiation, Parathyroid hormone, Cell Separation, Rats, Sprague-Dawley, Andrology, Endocrinology, stomatognathic system, Dentin, medicine, Animals, Orthopedics and Sports Medicine, RNA, Messenger, Cells, Cultured, Dental Pulp, Glycosaminoglycans, Receptor, Parathyroid Hormone, Type 1, Odontoblasts, biology, Cell Differentiation, Alkaline Phosphatase, DMP1, Rats, Reverse transcription polymerase chain reaction, stomatognathic diseases, medicine.anatomical_structure, Gene Expression Regulation, Biochemistry, Cell culture, biology.protein, Receptors, Parathyroid Hormone, Alkaline phosphatase |
| الوصف: | To establish a cell culture system that reflects the dentin formation in dental pulp tissue, we used dental pulp cells enzymatically isolated from rat incisor teeth. During the 20-day culture period, the cells exhibited various phenotypes of the odontoblast differentiation process, from the immature stage to the terminal mineralization stage. The cells began to form the mineralized nodules from day 10, and the nodules became larger by day 20. Alkaline phosphatase (ALP)-positive cells surrounded the mineralized nodules. The ALP activity in the cell layers was maximal on day 5, and gradually decreased to day 20. The calcium content in the cell layers was very low by day 10, and significantly increased from day 15. Sulfated glycosamino-glycans (GAGs) contained in the cell layers increased by day 15, but the content then decreased by day 20. The dental pulp cells produced a small amount of osteocalcin that was released into the culture medium by day 10, and the amount secreted increased markedly from day 15. The expression of osteocalcin and parathyroid hormone/parathyroid hormone-related peptide (PTH/PTHrP) receptor mRNA was evident as early as day 5, and the expression of each gradually increased with the number of days in culture. Dentin matrix protein (Dmp1) mRNA gene transcripts were identified by use of the reverse transcription polymerase chain reaction (RT-PCR) in the cells throughout the culture period. The present results demonstrate that this culture system is useful for studying the differentiation process of the odontoblast-like cells. |
| تدمد: | 1432-0827 0171-967X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aab647a62093dc8be46541304ef24d78Test https://doi.org/10.1007/s002230010028Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....aab647a62093dc8be46541304ef24d78 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14320827 0171967X |
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