Early diagnosis of bladder cancer through the detection of urinary tyrosine-phosphorylated proteins
| العنوان: | Early diagnosis of bladder cancer through the detection of urinary tyrosine-phosphorylated proteins |
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| المؤلفون: | Agata Notarpietro, Giuliana Giribaldi, Dario Fontana, Paolo Destefanis, Amina Khadjavi, Franca Mannu, Giovanni Fiorito, Paolo Vineis, Silvia Polidoro, Carlotta Sacerdote, Francesco Michelangelo Turrini, F Castagno, Paolo Gontero, Giuseppe Matullo, Antonino Battaglia, Marco Allasia, Antonella Pantaleo, Bruno Frea, Mauro Prato |
| المصدر: | British Journal of Cancer |
| بيانات النشر: | Nature Publishing Group, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Adult, Male, Cancer Research, medicine.medical_specialty, Pathology, Wilcoxon signed-rank test, Oncology, Urinary system, Pilot Projects, urinary tumour markers, Gastroenterology, Cohort Studies, diagnostic assay, Internal medicine, medicine, Biomarkers, Tumor, Humans, Molecular Diagnostics, Early Detection of Cancer, Aged, Neoplasm Staging, Aged, 80 and over, Bladder cancer, medicine.diagnostic_test, business.industry, Confounding, Case-control study, Cystoscopy, Middle Aged, Protein-Tyrosine Kinases, medicine.disease, urinary tyrosine-phosphorylated proteins, Phosphoproteins, Urinary Bladder Neoplasms, Case-Control Studies, Cohort, bladder cancer, Tyrosine, Female, business, Cohort study |
| الوصف: | Background: A noninvasive, highly sensitive and specific urine test is needed for bladder cancer (BC) diagnosis and surveillance in addition to the invasive cystoscopy. We previously described the diagnostic effectiveness of urinary tyrosine-phosphorylated proteins (UPY) and a new assay (UPY-A) for their measurement in a pilot study. The aim of this work was to evaluate the performances of the UPY-A using an independent cohort of 262 subjects. Methods: Urinary tyrosine-phosphorylated proteins were measured by UPY-A test. The area under ROC curve, cutoff, sensitivity, specificity and predictive values of UPY-A were determined. The association of UPY levels with tumour staging, grading, recurrence and progression risk was analysed by Kruskal–Wallis and Wilcoxon's test. To test the probability to be a case if positive at the UPY-A, a logistic test adjusted for possible confounding factor was used. Results: Results showed a significant difference of UPY levels between patients with BC vs healthy controls. For the best cutoff value, 261.26 Standard Units (SU), the sensitivity of the assay was 80.43% and the specificity was 78.82%. A statistically significant difference was found in the levels of UPY at different BC stages and grades between Ta and T1 and with different risk of recurrence and progression. A statistically significant increased risk for BC at UPY-A ⩾261.26 SU was observed. Conclusions: The present study supplies important information on the diagnostic characteristics of UPY-A revealing remarkable performances for early stages and allowing its potential use for different applications encompassing the screening of high-risk subjects, primary diagnosis and posttreatment surveillance. |
| اللغة: | English |
| تدمد: | 1532-1827 0007-0920 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2acd8ca1216bff068f3f43257092eaa8Test http://europepmc.org/articles/PMC4522638Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2acd8ca1216bff068f3f43257092eaa8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15321827 00070920 |
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