التفاصيل البيبلوغرافية
| العنوان: |
Identification of proteomic biomarkers predicting prostate cancer aggressiveness and lethality despite biopsy-sampling error. |
| المؤلفون: |
Shipitsin, M, Small, C, Choudhury, S, Giladi, E, Friedlander, S, Nardone, J, Hussain, S, Hurley, A D, Ernst, C, Huang, Y E, Chang, H, Nifong, T P, Rimm, D L, Dunyak, J, Loda, M, Berman, D M, Blume-Jensen, P |
| المصدر: |
British Journal of Cancer; 9/9/2014, Vol. 111 Issue 6, p1201-1212, 12p, 2 Diagrams, 3 Charts, 3 Graphs |
| مصطلحات موضوعية: |
PROTEOMICS, BIOMARKERS, BIOPSY, PROSTATE cancer, PROSTATECTOMY, SAMPLING errors |
| مستخلص: |
Background:Key challenges of biopsy-based determination of prostate cancer aggressiveness include tumour heterogeneity, biopsy-sampling error, and variations in biopsy interpretation. The resulting uncertainty in risk assessment leads to significant overtreatment, with associated costs and morbidity. We developed a performance-based strategy to identify protein biomarkers predictive of prostate cancer aggressiveness and lethality regardless of biopsy-sampling variation.Methods:Prostatectomy samples from a large patient cohort with long follow-up were blindly assessed by expert pathologists who identified the tissue regions with the highest and lowest Gleason grade from each patient. To simulate biopsy-sampling error, a core from a high- and a low-Gleason area from each patient sample was used to generate a 'high' and a 'low' tumour microarray, respectively.Results:Using a quantitative proteomics approach, we identified from 160 candidates 12 biomarkers that predicted prostate cancer aggressiveness (surgical Gleason and TNM stage) and lethal outcome robustly in both high- and low-Gleason areas. Conversely, a previously reported lethal outcome-predictive marker signature for prostatectomy tissue was unable to perform under circumstances of maximal sampling error.Conclusions:Our results have important implications for cancer biomarker discovery in general and development of a sampling error-resistant clinical biopsy test for prediction of prostate cancer aggressiveness. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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