Urinary cytokine profiles according to the site of blockade of the renin-angiotensin system in nephrectomized rats
| العنوان: | Urinary cytokine profiles according to the site of blockade of the renin-angiotensin system in nephrectomized rats |
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| المؤلفون: | Mauro M. Teixeira, Nilo César do Vale Baracho, Thiago Macedo e Cordeiro, Natalia Pessoa Rocha, Marconi Augusto Aguiar dos Reis, Victor Feracin, Regina Maria Pereira, Katia Daniela Silveira, Ana Cristina Simões e Silva |
| المصدر: | Brazilian Journal of Nephrology, Vol 39, Iss 2, Pp 108-118 Brazilian Journal of Nephrology v.39 n.2 2017 Jornal Brasileiro de Nefrologia Sociedade Brasileira de Nefrologia (SBN) instacron:SBN Jornal Brasileiro de Nefrologia, Volume: 39, Issue: 2, Pages: 108-118, Published: JUN 2017 |
| بيانات النشر: | Sociedade Brasileira de Nefrologia |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Angiotensin receptor, medicine.medical_treatment, angiotensinas, Tetrazoles, Angiotensin-Converting Enzyme Inhibitors, urologic and male genital diseases, lcsh:RC870-923, Nephrectomy, Renin-Angiotensin System, Random Allocation, chemistry.chemical_compound, kidney failure, chronic, 0302 clinical medicine, Enalapril, Fumarates, antagonistas de receptores de angiotensina, angiotensins, nephrectomy, nefrectomia, biology, General Medicine, angiotensin-converting enzyme inhibitors, 030220 oncology & carcinogenesis, Cytokines, medicine.drug, medicine.medical_specialty, Renal function, 03 medical and health sciences, Internal medicine, medicine, angiotensin receptor antagonists, Animals, Rats, Wistar, business.industry, Biphenyl Compounds, Angiotensin-converting enzyme, Aliskiren, medicine.disease, lcsh:Diseases of the genitourinary system. Urology, Amides, Rats, Candesartan, 030104 developmental biology, Endocrinology, chemistry, falência renal crônica, inibidores da enzima conversora de angiotensina, biology.protein, Benzimidazoles, business, Angiotensin II Type 1 Receptor Blockers, Kidney disease |
| الوصف: | Introduction: It is still unknown how the pharmacological inhibition of the Renin Angiotensin System (RAS) impacts the levels of inflammation and fibrosis biomarkers. Objective: This study sought to evaluate the effect of enalapril, candesartan and aliskiren on urinary levels of cytokines in a model of chronic kidney disease (CKD). Methods: Male Wistar rats were submitted to surgical removal of ¾ of renal parenchyma to induce CKD (¾ nephrectomy), or subjected to sham surgery (control). Animals were then randomized into five groups: Sham surgery receiving vehicle; ¾ Nephrectomy receiving vehicle; ¾ Nephrectomy receiving enalapril (10 mg/kg); ¾ Nephrectomy receiving candesartan (10 mg/kg) and ¾ Nephrectomy receiving aliskiren (10 mg/kg). Urine output, water intake, mean arterial pressure (MAP) and urinary concentrations of creatinine, urea, albuminuria, Na+, K+, interleukin (IL) -1β, IL-6, IL-10 and transforming growth factor beta (TGF-β) were measured. Results: Nephrectomy significantly impaired renal function, increased MAP and altered the levels of all evaluated cytokines in urine. Enalapril, candesartan and aliskiren improved renal function and decreased MAP and IL-6 when compared to vehicle-treated nephrectomized group. Candesartan and aliskiren decreased IL-1β, while only candesartan reduced TGF-β and only aliskiren increased IL-10. Conclusion: Enalapril, candesartan and aliskiren presented similar effects on improving renal function and reducing MAP and urinary levels of IL-6 in rats with CKD. On the other hand, cytokine profile differed according to the treatment, suggesting that differential mechanisms were triggered in response to the site of RAS blockade. Resumo Introdução: Ainda não se sabe como a inibição farmacológica do Sistema Renina Angiotensina (SRA) afeta os níveis de biomarcadores de inflamação e fibrose. Objetivo: Este estudo pretendeu avaliar o efeito de enalapril, candesartan e alisquireno sobre os níveis urinários de citocinas em um modelo de doença renal crônica (DRC). Métodos: Ratos Wistar machos foram submetidos à remoção cirúrgica de ¾ do parênquima renal para induzir DRC (nefrectomia), ou submetidos à cirurgia fictícia (controle). Animais foram então randomizados em cinco grupos: Cirurgia fictícia recebendo veículo; Nefrectomia recebendo veículo; Nefrectomia recebendo enalapril (10 mg/kg); Nefrectomia recebendo candesartan (10 mg/kg) e Nefrectomia recebendo alisquireno (10 mg/kg). Débito urinário, ingesta hídrica, pressão arterial media (PAM) e concentrações urinárias de creatinina, ureia, albumina, Na+, K+, interleucina (IL) -1β, IL-6, IL-10 e fator de transformação e crescimento beta (TGF-β) foram medidas. Resultados: A nefrectomia comprometeu significativamente a função renal, aumentou a PAM e alterou os níveis de todas as citocinas avaliadas na urina. Enalapril, candesartan e alisquireno melhoraram a função renal e diminuíram a PAM e a IL-6 quando comparado aos grupo de animais nefrectomizados tratados com veículo. Candesartan e alisquireno reduziram IL-1β, enquanto somente candesartan diminuiu o TGF-β e somente alisquireno aumentou a IL-10. Conclusão: Enalapril, candesartan e alisquireno apresentaram efeitos similares em relação à melhora da função renal e redução da PAM e dos níveis urinários de IL-6 em ratos com DRC. Por outro lado, o perfil de citocinas diferiu de acordo com o tratamento, sugerindo que diferentes mecanismos sejam desencadeados em resposta ao local de bloqueio do SRA. |
| وصف الملف: | text/html |
| اللغة: | English |
| تدمد: | 2175-8239 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::98d4a7aa9792a5fd1074f29190750016Test http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-28002017000200108&lng=en&tlng=enTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....98d4a7aa9792a5fd1074f29190750016 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21758239 |
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