Biofilm production and beta-lactamic resistance in Brazilian Staphylococcus aureus isolates from bovine mastitis
| العنوان: | Biofilm production and beta-lactamic resistance in Brazilian Staphylococcus aureus isolates from bovine mastitis |
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| المؤلفون: | Irene da Silva Coelho, Dayanne Araújo de Melo, Helene Santos Barbosa, Miliane Moreira Soares de Souza, Bianca da Silva Soares, Cássia Couto da Motta, Shana de Mattos de Oliveira Coelho, Viviane F. Marques |
| المصدر: | Brazilian Journal of Microbiology, Vol 48, Iss 1, Pp 118-124 Brazilian Journal of Microbiology Brazilian Journal of Microbiology, Volume: 48, Issue: 1, Pages: 118-124, Published: MAR 2017 Brazilian Journal of Microbiology v.48 n.1 2017 Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
| بيانات النشر: | Sociedade Brasileira de Microbiologia |
| مصطلحات موضوعية: | 0301 basic medicine, Proteomics, Staphylococcus aureus, Proteome, RNAIII, Virulence Factors, 030106 microbiology, Veterinary Microbiology, lcsh:QR1-502, Virulence, Drug resistance, Microbial Sensitivity Tests, Mastitis, Biology, medicine.disease_cause, Antimicrobial resistance, Microbiology, beta-Lactam Resistance, lcsh:Microbiology, 03 medical and health sciences, Antibiotic resistance, Bacterial Proteins, medicine, Animals, Mastitis, Bovine, Genetic Association Studies, Minimum bactericidal concentration, Biofilm, Staphylococcal Infections, biochemical phenomena, metabolism, and nutrition, Anti-Bacterial Agents, Biofilms, Agr types, Trans-Activators, bacteria, Cattle, Female, Staphylococcus |
| الوصف: | Staphylococcus spp. play an important role in the etiology of bovine mastitis. Staphylococcus aureus is considered the most relevant species due to the production of virulence factors such as slime, which is required for biofilm formation. This study aimed to evaluate biofilm production and its possible relation to beta-lactamic resistance in 20 S. aureus isolates from bovine mastitic milk. The isolates were characterized by pheno-genotypic and MALDI TOF-MS assays and tested for genes such as icaA, icaD, bap, agr RNAIII, agr I, agr II, agr III, and agr IV, which are related to slime production and its regulation. Biofilm production in microplates was evaluated considering the intervals determined along the bacterial growth curve. In addition, to determine the most suitable time interval for biofilm analysis, scanning electron microscopy was performed. Furthermore, genes such as mecA and blaZ that are related to beta-lactamic resistance and oxacillin susceptibility were tested. All the studied isolates were biofilm producers and mostly presented icaA and icaD. The Agr type II genes were significantly prevalent. According to the SEM, gradual changes in the bacterial arrangement were observed during biofilm formation along the growth curve phases, and the peak was reached at the stationary phase. In this study, the penicillin resistance was related to the production of beta-lactamase, and the high minimal bactericidal concentration for cefoxitin was possibly associated with biofilm protection. Therefore, further studies are warranted to better understand biofilm formation, possibly contributing to our knowledge about bacterial resistance in vivo. |
| وصف الملف: | text/html |
| اللغة: | English |
| تدمد: | 1678-4405 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::88e1f5eaa26796885b6b5976d86abc8eTest http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100118&lng=en&tlng=enTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....88e1f5eaa26796885b6b5976d86abc8e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16784405 |
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