Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk
| العنوان: | Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk |
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| المؤلفون: | Patrícia Yoshida Faccioli-Martins, Maria de Lourdes Ribeiro de Souza da Cunha, Simone Fernandes, Ariane Dantas, Danilo Flávio Moraes Riboli, Katheryne Benini Martins, Valéria Cataneli Pereira, Aline Aparecida de Oliveira, Luiz Francisco Zafalon |
| المساهمون: | Universidade Estadual Paulista (Unesp), Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
| المصدر: | Brazilian Journal of Microbiology, Vol 46, Iss 2, Pp 535-543 (2015) Brazilian Journal of Microbiology, Volume: 46, Issue: 2, Pages: 535-543, Published: JUN 2015 Brazilian Journal of Microbiology SciELO Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Brazilian Journal of Microbiology v.46 n.2 2015 Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
| بيانات النشر: | Sociedade Brasileira de Microbiologia, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | sheep, Staphylococcus aureus, antibiotic resistance, Veterinary Microbiology, virulence factors, lcsh:QR1-502, Virulence, Microbial Sensitivity Tests, Biology, medicine.disease_cause, mastitis, Microbiology, Virulence factor, California, lcsh:Microbiology, Antibiotic resistance, Media Technology, medicine, Animals, Reverse Transcriptase Polymerase Chain Reaction, SCCmec, Gene Expression Profiling, Biofilm, biochemical phenomena, metabolism, and nutrition, medicine.disease, Virology, Mastitis, Anti-Bacterial Agents, Electrophoresis, Gel, Pulsed-Field, Molecular Typing, Milk, Biofilms, California mastitis test |
| الوصف: | Made available in DSpace on 2015-08-26T19:19:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-06-01. Added 1 bitstream(s) on 2015-08-31T13:04:46Z : No. of bitstreams: 1 S1517-83822015000200535.pdf: 774594 bytes, checksum: eb7a895cff0c3bf9a22909d136c7769e (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The objective of this study was to characterize the clonal profile, virulence factors and antimicrobial resistance, particularly oxacillin resistance, of Staphylococcus aureus isolated from sheep milk. Milk samples were collected from all teats for the California Mastitis Test (CMT), somatic cell count, identification of S. aureus, investigation in these strains of genes encoding toxins (sea, seb, sec, sed, tst), biofilm (icaA, icaC, icaD, bap), leukocidin (luk-PV) oxacillin resistance by mecA gene detection and susceptibility testing (12 antibiotics). Messenger RNA expression was evaluated by RT-PCR in isolates carrying toxin and biofilm genes. Biofilm formation was also evaluated phenotypically by adherence to polystyrene plates. The clonal profile of S. aureus was investigated by pulsed-field gel electrophoresis. A total of 473 milk samples were collected from 242 animals on three farms and 20 S. aureus strains were isolated and none carried the mecA gene. The two sec gene-positive isolates and the isolates carrying the tst and luk-PV genes were positive by RT-PCR. Staphylococcus aureus isolated from the three flocks studied showed high susceptibility to the drugs tested and none was biofilm producer, indicating that biofilm formation was not a virulence factor causing infection by these strains. The typing of 17 S. aureus isolates revealed the presence of a common clone on the three farms studied, and the presence and expression of the sec and tst genes in one strain of this clone suggest the possible acquisition of virulence genes by this clone, a fact that is important for animal health and food hygiene. Universidade Estadual Paulista Instituto de Biociências Departamento de Microbiologia e Imunologia Universidade Estadual Paulista Faculdade de Medicina Veterinária e Zootecnia Departamento de Produção Animal Embrapa Pecuária Sudeste Universidade Estadual Paulista Instituto de Biociências Departamento de Microbiologia e Imunologia Universidade Estadual Paulista Faculdade de Medicina Veterinária e Zootecnia Departamento de Produção Animal |
| وصف الملف: | text/html |
| اللغة: | English |
| تدمد: | 1678-4405 1517-8382 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::45b8b035d838acbac7d621242879c346Test http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200535&lng=en&tlng=enTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....45b8b035d838acbac7d621242879c346 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16784405 15178382 |
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