Trueperella pyogenes pyolysin inhibits lipopolysaccharide-induced inflammatory response in endometrium stromal cells via autophagy- and ATF6-dependent mechanism
| العنوان: | Trueperella pyogenes pyolysin inhibits lipopolysaccharide-induced inflammatory response in endometrium stromal cells via autophagy- and ATF6-dependent mechanism |
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| المؤلفون: | Maozhen Qi, Jianguo Liu, Huatao Chen, Aihua Wang, Pengfei Lin, Hongyu Niu, Xinyu Wang, Dong Zhou, Qingran Jiang, Yaping Jin |
| المصدر: | Braz J Microbiol |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Lipopolysaccharides, Cell signaling, Stromal cell, Lipopolysaccharide, Inflammasomes, Bacterial Toxins, ved/biology.organism_classification_rank.species, Biology, Microbiology, Virulence factor, Endometrium, Hemolysin Proteins, 03 medical and health sciences, chemistry.chemical_compound, Bacterial Proteins, NLR Family, Pyrin Domain-Containing 3 Protein, Autophagy, Media Technology, Trueperella pyogenes, Animals, 030304 developmental biology, Inflammation, 0303 health sciences, Innate immune system, 030306 microbiology, ATF6, ved/biology, Goats, Veterinary Microbiology - Research Paper, Recombinant Proteins, Activating Transcription Factor 6, Cell biology, chemistry, Actinomycetaceae, Female, Inflammation Mediators, Stromal Cells |
| الوصف: | Trueperella pyogenes (T. pyogenes) is a common opportunistic pathogen of many livestock and play an important regulation role during multibacterial infection and interaction with the host by its primary virulence factor pyolysin (PLO). The purpose of this study was to investigate the regulation role of PLO which serve as a combinational pathogen with lipopolysaccharide (LPS) during endometritis. In this study, the expression of bioactive recombinant PLO (rPLO) in a prokaryotic expression system and its purification are described. Moreover, we observed that rPLO inhibited the innate immune response triggered by LPS and that methyl-β-cyclodextrin (MBCD) abrogated this inhibitory effect in goat endometrium stromal cells (gESCs). Additionally, we show from pharmacological and genetic studies that rPLO-induced autophagy represses gene expression by inhibiting NLRP3 inflammasome activation. Importantly, this study reported that ATF6 serves as a primary regulator of the cellular inflammatory reaction to rPLO. Overall, these observations suggest that T. pyogenes PLO could create an immunosuppressive environment for other pathogens invasion by regulating cellular signaling pathways. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s42770-021-00422-5. |
| تدمد: | 1678-4405 1517-8382 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::75c187e7a07630e18aceaaf6a50b2d9fTest https://doi.org/10.1007/s42770-021-00422-5Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....75c187e7a07630e18aceaaf6a50b2d9f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16784405 15178382 |
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