Distribution and ultrastructural localization of the glucagon-like peptide-1 receptor (GLP-1R) in the rat brain
| العنوان: | Distribution and ultrastructural localization of the glucagon-like peptide-1 receptor (GLP-1R) in the rat brain |
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| المؤلفون: | Yvette Ruska, Balázs Gereben, Anett Szilvásy-Szabó, Morten Grønbech Rasch, Lotte Bjerre Knudsen, Charles Pyke, Richárd Sinkó, Thomas Egebjerg, Erzsébet Farkas, Csaba Fekete |
| المصدر: | Brain Structure & Function Brain Structure and Function |
| مصطلحات موضوعية: | 0301 basic medicine, Male, endocrine system, Histology, Immunocytochemistry, Axonal localization, 030209 endocrinology & metabolism, GLP-1 receptor, Distribution, Glucagon-Like Peptide-1 Receptor, Rats, Sprague-Dawley, 03 medical and health sciences, 0302 clinical medicine, Arcuate nucleus, medicine, Electron microscopy, Glucose homeostasis, Animals, Axon, Circumventricular organs, Neurons, Arc (protein), Chemistry, General Neuroscience, digestive, oral, and skin physiology, Brain, Immunohistochemistry, Cell biology, Rats, 030104 developmental biology, medicine.anatomical_structure, Original Article, Brainstem, Anatomy, Nucleus, hormones, hormone substitutes, and hormone antagonists |
| الوصف: | Glucagon-like peptide-1 (GLP-1) inhibits food intake and regulates glucose homeostasis. These actions are at least partly mediated by central GLP-1 receptor (GLP-1R). Little information is available, however, about the subcellular localization and the distribution of the GLP-1R protein in the rat brain. To determine the localization of GLP-1R protein in the rat brain, immunocytochemistry was performed at light and electron microscopic levels. The highest density of GLP-1R-immunoreactivity was observed in the circumventricular organs and regions in the vicinity of these areas like in the arcuate nucleus (ARC) and in the nucleus tractus solitarii (NTS). In addition, GLP-1R-immunreactive (IR) neuronal profiles were also observed in a number of telencephalic, diencephalic and brainstem areas and also in the cerebellum. Ultrastructural examination of GLP-1R-immunoreactivity in energy homeostasis related regions showed that GLP-1R immunoreactivity is associated with the membrane of perikarya and dendrites but GLP-1R can also be observed inside and on the surface of axon varicosities and axon terminals. In conclusion, in this study we provide a detailed map of the GLP-1R-IR structures in the CNS. Furthermore, we demonstrate that in addition to the perikaryonal and dendritic distribution, GLP-1R is also present in axonal profiles suggesting a presynaptic action of GLP-1. The very high concentration of GLP-1R-profiles in the circumventricular organs and in the ARC and NTS suggests that peripheral GLP-1 may influence brain functions via these brain areas. Supplementary Information The online version contains supplementary material available at 10.1007/s00429-020-02189-1. |
| اللغة: | English |
| تدمد: | 1863-2661 1863-2653 |
| DOI: | 10.1007/s00429-020-02189-1 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c60fb6e5e6bbfc4a323ab88a196cb45aTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c60fb6e5e6bbfc4a323ab88a196cb45a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18632661 18632653 |
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| DOI: | 10.1007/s00429-020-02189-1 |