Evidence of Reelin Signaling in GBM and Its Derived Cancer Stem Cells
| العنوان: | Evidence of Reelin Signaling in GBM and Its Derived Cancer Stem Cells |
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| المؤلفون: | Gigliola Sica, Alessio D’Alessio, Filippo Biamonte, Antonio Filippini |
| المصدر: | Brain Sciences, Vol 11, Iss 745, p 745 (2021) Brain Sciences |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | cancer stem cells, medicine.medical_treatment, Population, Central nervous system, glioblastoma, peritumoral tissue, reelin, Neurosciences. Biological psychiatry. Neuropsychiatry, 03 medical and health sciences, 0302 clinical medicine, Cancer stem cell, medicine, Reelin, education, 030304 developmental biology, 0303 health sciences, education.field_of_study, biology, Brief Report, General Neuroscience, DAB1, Radiation therapy, medicine.anatomical_structure, nervous system, Cell culture, biology.protein, Cancer research, Immunohistochemistry, Settore BIO/17 - ISTOLOGIA, 030217 neurology & neurosurgery, RC321-571 |
| الوصف: | Glioblastoma (GBM) is the most aggressive and malignant form of primary brain cancer, characterized by an overall survival time ranging from 12 to 18 months. Despite the progress in the clinical treatment and the growing number of experimental data aimed at investigating the molecular bases of GBM development, the disease remains characterized by a poor prognosis. Recent studies have proposed the existence of a population of GBM cancer stem cells (CSCs) endowed with self-renewal capability and a high tumorigenic potential that are believed to be responsible for the resistance against common chemotherapy and radiotherapy treatments. Reelin is a large secreted extracellular matrix glycoprotein, which contributes to positioning, migration, and laminar organization of several central nervous system structures during brain development. Mutations of the reelin gene have been linked to disorganization of brain structures during development and behavioral anomalies. In this study, we explored the expression of reelin in GBM and its related peritumoral tissue and performed the same analysis in CSCs isolated from both GBM (GCSCs) and peritumoral tissue (PCSCs) of human patients. Our findings reveal (i) the higher expression of reelin in GBM compared to the peritumoral tissue by immunohistochemical analysis, (ii) the mRNA expression of both reelin and its adaptor molecule Dab1 in either CSC subtypes, although at a different extent; and (iii) the contribution of CSCs-derived reelin in the migration of human primary GBM cell line U87MG. Taken together, our data indicate that the expression of reelin in GBM may represent a potential contribution to the regulation of GBM cancer stem cells behavior, further stimulating the interest on the reelin pathway as a potential target for GBM treatment. |
| تدمد: | 2076-3425 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d0629a6244c06a2c593aab1617c10ffTest https://doi.org/10.3390/brainsci11060745Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6d0629a6244c06a2c593aab1617c10ff |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20763425 |
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