3D-printed titanium implant combined with interleukin 4 regulates ordered macrophage polarization to promote bone regeneration and angiogenesis
| العنوان: | 3D-printed titanium implant combined with interleukin 4 regulates ordered macrophage polarization to promote bone regeneration and angiogenesis |
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| المؤلفون: | Hongwei Wang, Lei Cheng, Shuaishuai Cao, Mingzhi Yu, Florian M. Thieringer, Dong Zhang, Yi Wan, Bing Ren, Da-Wang Zhao, Yuan-Hua Sang, Chao Liu, Xiao Zhang |
| المصدر: | Bone & Joint Research Bone & Joint Research, Vol 10, Iss 7, Pp 411-424 (2021) |
| بيانات النشر: | British Editorial Society of Bone & Joint Surgery, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0303 health sciences, 3d printed, Titanium implant, Angiogenesis, Chemistry, macrophage polarization, Macrophage polarization, Diseases of the musculoskeletal system, 02 engineering and technology, 021001 nanoscience & nanotechnology, Interleukin 4, Cell biology, Biomaterials, 03 medical and health sciences, RC925-935, Macrophage, Orthopedics and Sports Medicine, Surgery, Immune reaction, 3D-printed titanium implant, 0210 nano-technology, Bone regeneration, 030304 developmental biology |
| الوصف: | Aims The use of 3D-printed titanium implant (DT) can effectively guide bone regeneration. DT triggers a continuous host immune reaction, including macrophage type 1 polarization, that resists osseointegration. Interleukin 4 (IL4) is a specific cytokine modulating osteogenic capability that switches macrophage polarization type 1 to type 2, and this switch favours bone regeneration. Methods IL4 at concentrations of 0, 30, and 100 ng/ml was used at day 3 to create a biomimetic environment for bone marrow mesenchymal stromal cell (BMMSC) osteogenesis and macrophage polarization on the DT. The osteogenic and immune responses of BMMSCs and macrophages were evaluated respectively. Results DT plus 30 ng/ml of IL4 (DT + 30 IL4) from day 3 to day 7 significantly (p < 0.01) enhanced macrophage type 2 polarization and BMMSC osteogenesis compared with the other groups. Local injection of IL4 enhanced new bone formation surrounding the DT. Conclusion DT + 30 IL4 may switch macrophage polarization at the appropriate timepoints to promote bone regeneration. Cite this article: Bone Joint Res 2021;10(7):411–424. |
| تدمد: | 2046-3758 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::61318eef3203187f93d2b93b23e13346Test https://doi.org/10.1302/2046-3758.107.bjr-2020-0334.r4Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....61318eef3203187f93d2b93b23e13346 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20463758 |
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