| مستخلص: |
Identify new potential biomarkers of osteoporosis at an early stage, by magnetic resonance spectroscopy (MRS), studying early changes in the metabolic profile of bone-marrow fatty acids in women's calcanei during healthy aging and osteoporosis status. Single voxel MRS was performed by using a point resolved spectroscopy (PRESS) sequence at 3T. Thirty-four Caucasian women (age range: 22–59 years) were recruited to investigate calcaneus bone marrow. The cohort was constituted of four groups according to age, menopausal status, and T-score evaluated after a DXA examination on the femoral neck. Women were classified in young control (n = 11, mean age = 26.5 ± 3.8 y, age range: 22–34 years), perimenopausal groups (n = 11, mean age = 42.0 ± 3.6 y, age range: 37–47 years), postmenopausal group (n = 9, mean age = 55.4 ± 2.9 y, age range: 50–59 years, mean T-score = −1.70 ± 0.50) and osteoporotic group (n = 6, mean age = 53.0 ± 2.8 y, age range: 50–58 years, mean T-score = −2.54 ± 0.10). The total lipid content (TL), the Unsaturation Index (UI), and the fraction of unsaturated/polyunsaturated fatty acid (f UFA and f PUFA) were calculated. TL was significantly correlated with age (r = 0. 73, p < 0. 001). TL increases linearly with age in the young + perimenopausal population (r = 0. 92, p < 0. 001) but this trend is not significant in the postmenopausal subject (r = 0. 48, p = 0. 07). No significant correlation was found between T-Score and TL in postmenopausal and osteoporotic women, whereas a significant correlation was found between TL and time interval (t p) between the age at menopause and the age of the subject at the MRS examination. Conversely, no correlation was found between T-score and t p. The unsaturation index (UI) does not significantly discriminate between osteoporotic, peri- and postmenopausal women. On the other hand, f UFA is significantly different in peri-menopausal and osteoporotic subjects (p = 0. 02), while f PUFA is significantly different both between peri- and postmenopausal women (p = 0. 05) and postmenopausal and osteoporotic subjects (p = 0. 03). Both f UFA and f PUFA did not correlate with subjects' age. In the female calcaneus, f UFA and f PUFA are promising measurable quantities for the characterization of bone marrow's composition potentially correlated with the development of osteoporosis, whereas UI does not differentiate between subjects of varying osteoporotic status. The fact that the TL in the calcaneus is correlated with t p , indicates that active metabolic changes are still occurring in these subjects, giving complementary information to the DXA about the changes in bone marrow's composition which may affect the whole bone health. • Calcaneal fUFA and fPUFA are promising markers for bone metabolism. • Total lipids correlates with the interval time from the menopausal age. • Fats quantification is complementary to the DXA for establish bone status. [ABSTRACT FROM AUTHOR] |
