Spatial expression of glucagon-like peptide 1 receptor and caveolin-1 in hepatocytes with macrovesicular steatosis in non-alcoholic steatohepatitis
| العنوان: | Spatial expression of glucagon-like peptide 1 receptor and caveolin-1 in hepatocytes with macrovesicular steatosis in non-alcoholic steatohepatitis |
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| المؤلفون: | Wataru Ando, Hiroaki Yokomori |
| المصدر: | BMJ Open Gastroenterology, Vol 7, Iss 1 (2020) BMJ Open Gastroenterology |
| بيانات النشر: | BMJ Publishing Group, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Liver Cirrhosis, Pathology, medicine.medical_specialty, endocrine system, Immunoelectron microscopy, Caveolin 1, 030209 endocrinology & metabolism, Case Report, liver, Glucagon-Like Peptide-1 Receptor, Monocytes, End Stage Liver Disease, 03 medical and health sciences, Liver disease, 0302 clinical medicine, Non-alcoholic Fatty Liver Disease, Lipid droplet, Caveolae, Caveolin, Medicine, Humans, hepatitis, nonalcoholic steatohepatitis, lcsh:RC799-869, Microscopy, Immunoelectron, business.industry, Endoplasmic reticulum, Liver Neoplasms, Gastroenterology, medicine.disease, Immunohistochemistry, 030104 developmental biology, Hepatocytes, liver immunology, lcsh:Diseases of the digestive system. Gastroenterology, Steatohepatitis, business |
| الوصف: | ObjectiveNon-alcoholic steatohepatitis (NASH) can progress to fibrosis, cirrhosis and end-stage liver disease. Glucagon-like peptide 1 receptor (GLP-1R) mediates β cell function. Its receptor agonists, currently used to treat type 2 diabetes mellitus, might be effective against NASH. GLP-1R, a G protein-coupled receptor family member, preferentially localises to caveolae. Therefore, we ascertained the cellular localisation of GLP-1R and caveolin (CAV)-1 in NASH liver.MethodsLiver biopsies were obtained from three patients with NASH and were compared with those of four normal patients. Immunohistochemistry (IHC) and immunoelectron microscopy (IEM) were used to compare GLP-1R and CAV-1 expression in the livers of patients with metastatic liver cancer and normal patients.ResultsIHC showed that GLP-1R localised to basolateral membranes of hepatocytes with macrovesicular steatosis and was expressed in monocytes infiltrating hepatic sinusoids. CAV-1 was minimally associated with low-electron density lipid droplets (LDs) in hepatocytes. IEM showed small clusters of GLP-1R molecules on the peripheral rims of LDs and on cytoplasmic leaflets of endoplasmic reticulum membranes and vesicles, whereas CAV-1 molecules were found in LD caveolae.ConclusionsGLP-1R is present in the lipid microdomains of hepatocytes with macrovesicular steatosis. These results may help inform future studies about the liver-specific mechanisms of GLP-1 modulation in NASH therapy. |
| اللغة: | English |
| تدمد: | 2054-4774 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11ec04d3eb4fc00bd2457231b3a0123fTest https://bmjopengastro.bmj.com/content/7/1/e000370.fullTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....11ec04d3eb4fc00bd2457231b3a0123f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20544774 |
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