Neurofilament light oligomers in neurodegenerative diseases: quantification by homogeneous immunoassay in cerebrospinal fluid

التفاصيل البيبلوغرافية
العنوان:	Neurofilament light oligomers in neurodegenerative diseases: quantification by homogeneous immunoassay in cerebrospinal fluid
المؤلفون:	Henrik Zetterberg, Kaj Blennow, Aitana Sogorb-Esteve, Ingmar Skoog, Ulf Andreasson, Silke Kern, Kathryn Knowles, Francisco J. Meda, Imogen J. Swift, Jonathan D. Rohrer, Anna Dittrich, Bruno Becker, Hlin Kvartsberg
المصدر:	BMJ Neurology Open, Vol 5, Iss 1 (2023)
بيانات النشر:	BMJ Publishing Group, 2023.
سنة النشر:	2023
المجموعة:	LCC:Neurosciences. Biological psychiatry. Neuropsychiatry
مصطلحات موضوعية:	Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571
الوصف:	Background Neurofilament light (NfL) is a widely used biomarker for neurodegeneration. NfL is prone to oligomerisation, but available assays do not reveal the exact molecular nature of the protein variant measured. The objective of this study was to develop a homogeneous ELISA capable of quantifying oligomeric NfL (oNfL) in cerebrospinal fluid (CSF).Methods A homogeneous ELISA, based on the same capture and detection antibody (NfL21), was developed and used to quantify oNfL in samples from patients with behavioural variant frontotemporal dementia (bvFTD, n=28), non-fluent variant primary progressive aphasia (nfvPPA, n=23), semantic variant PPA (svPPA, n=10), Alzheimer’s disease (AD, n=20) and healthy controls (n=20). The nature of NfL in CSF, and the recombinant protein calibrator, was also characterised by size exclusion chromatography (SEC).Results CSF concentration of oNfL was significantly higher in nfvPPA (p
نوع الوثيقة:	article
وصف الملف:	electronic resource
اللغة:	English
تدمد:	2632-6140 81939248
العلاقة:	https://neurologyopen.bmj.com/content/5/1/e000395.fullTest; https://doaj.org/toc/2632-6140Test
DOI:	10.1136/bmjno-2022-000395
الوصول الحر:	https://doaj.org/article/091f8c81939248249208b4a75beb258fTest
رقم الانضمام:	edsdoj.091f8c81939248249208b4a75beb258f
قاعدة البيانات:	Directory of Open Access Journals

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تدمد:	26326140 81939248
DOI:	10.1136/bmjno-2022-000395