E-test versus agar dilution for antibiotic susceptibility testing of Helicobacter pylori: a comparison study
| العنوان: | E-test versus agar dilution for antibiotic susceptibility testing of Helicobacter pylori: a comparison study |
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| المؤلفون: | Ali Khomsan, Kartika Afrida Fauzia, I Ketut Adnyana, Neneng Ratnasari, Poernomo Boedi Setiawan, Ari Fahrial Syam, Langgeng Agung Waskito, Muhammad Miftahussurur, Iswan Abbas Nusi, Dalla Doohan, Junko Akada, Yoshio Yamaoka |
| المصدر: | BMC Research Notes BMC Research Notes, Vol 13, Iss 1, Pp 1-6 (2020) |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, medicine.medical_specialty, Tetracycline, medicine.drug_class, Antibiotics, lcsh:Medicine, Microbial Sensitivity Tests, Gastroenterology, General Biochemistry, Genetics and Molecular Biology, Agar dilution, 03 medical and health sciences, 0302 clinical medicine, Bias, Levofloxacin, Internal medicine, Clarithromycin, Drug Resistance, Bacterial, medicine, lcsh:Science (General), lcsh:QH301-705.5, biology, Helicobacter pylori, business.industry, E-test, lcsh:R, General Medicine, Amoxicillin, biology.organism_classification, 3. Good health, Metronidazole, Agar, Research Note, 030104 developmental biology, lcsh:Biology (General), 030211 gastroenterology & hepatology, business, lcsh:Q1-390, medicine.drug, Antibiotic susceptibility test |
| الوصف: | ObjectiveFor evaluating the antibiotic resistance ofHelicobacter pylori, the agar dilution method is the gold standard; however, using this method in daily practice is laborious. E-test has been proposed to be an uncomplicated method. This study was aimed at validating the E-test and detecting the presence of any bias between the agar dilution method and E-test.ResultsThe agar dilution method and E-test were performed using five antibiotics for 72 strains ofH. pyloriobtained from clinical patients in Indonesia. The E-test’s results showed a higher prevalence of resistance to all the antibiotics tested but the difference was not significant. Results showed high essential agreement (> 90.0%) for all the antibiotics, but only 84.7% for metronidazole. The agreement for MIC value was acceptable for levofloxacin, clarithromycin, and metronidazole. For amoxicillin, it showed only fair agreement (0.25) by the Kappa analysis and significant difference by Passing-Bablok regression. Even though some discrepancies were found, the E-test has an acceptable agreement for levofloxacin, metronidazole, tetracycline, and clarithromycin but further confirmation may be necessary for amoxicillin. |
| تدمد: | 1756-0500 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af02e6ab71a12161c0ce61a5af3251dfTest https://pubmed.ncbi.nlm.nih.gov/31924273Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....af02e6ab71a12161c0ce61a5af3251df |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17560500 |
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