The importance of the PD-1/PD-L1 pathway at the maternal-fetal interface
| العنوان: | The importance of the PD-1/PD-L1 pathway at the maternal-fetal interface |
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| المؤلفون: | Laszlo Szereday, Nelli Farkas, Matyas Meggyes, Eva Miko, Brigitta Szigeti |
| المصدر: | BMC Pregnancy and Childbirth, Vol 19, Iss 1, Pp 1-12 (2019) BMC Pregnancy and Childbirth |
| بيانات النشر: | BMC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Adult, CD4-Positive T-Lymphocytes, PD-L1, Programmed Cell Death 1 Receptor, chemical and pharmacologic phenomena, CD8-Positive T-Lymphocytes, Peripheral blood mononuclear cell, lcsh:Gynecology and obstetrics, Antibodies, B7-H1 Antigen, Histocompatibility, Maternal-Fetal, Flow cytometry, Andrology, 03 medical and health sciences, Maternal immunotolerance, 0302 clinical medicine, Immune system, Lysosomal-Associated Membrane Protein 1, Pregnancy, PD-1, Decidua, Humans, Medicine, Cytotoxic T cell, 030212 general & internal medicine, Receptor, lcsh:RG1-991, 030219 obstetrics & reproductive medicine, biology, medicine.diagnostic_test, business.industry, Obstetrics and Gynecology, Flow Cytometry, NKG2D, Maternal-fetal interface, NK Cell Lectin-Like Receptor Subfamily K, Leukocytes, Mononuclear, biology.protein, Female, business, CD8, Signal Transduction, Research Article |
| الوصف: | Background Our goal with this study was to investigate the contribution of PD-1/PD-L1 immune-checkpoint pathway to maternal immunotolerance mechanisms. Methods Thirteen healthy pregnant women and 10 non-pregnant controls were involved in this project. PBMCs and DICs were isolated from peripheral blood and from decidual tissues. After the characterization of different immune cell subsets, we used fluorochrome-conjugated monoclonal antibodies to measure the expression level of PD-1, PD-L1, NKG2D, and CD107a molecules by flow cytometry. Results We measured significant alternations in the proportion of decidual immune cell subsets compared to the periphery. Elevated PD-1 expression by decidual CD8+ T, CD4+ T, and NKT-like cells were also detected accompanied by the increased PD-L1 expression by decidual CD4+ T, Treg, NKT-like and CD56 + NK cell subsets compared to peripheral blood. The cytotoxic potential was significantly higher in PD-1- decidual immune cells compared to the periphery, however we measured a significantly lower cytotoxicity in the decidual PD-1+ CD8+ T cells compared with the peripheral subsets. An activation receptor NKG2D expression was decreased by the PD-1+ CD8+ T subsets in the first trimester compared to non-pregnant condition but the expression level of the decidual counterparts was significantly elevated compared to the periphery. The cytotoxic potential of decidual PD1/NKG2D double positive CD8+ T cells was significantly decreased compared to the peripheral subsets. Conclusions Based on our results we assume that PD-1/PD-L1 pathway might have a novel role in the maintaining of the local immunological environment. Accompanied by NKG2D activating receptor this checkpoint interaction could regulate decidual CD8 Tc cell subsets and may contribute maternal immunotolerance. |
| اللغة: | English |
| تدمد: | 1471-2393 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e9ac93ac701282a4b92d1d8bb1833f18Test http://link.springer.com/article/10.1186/s12884-019-2218-6Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e9ac93ac701282a4b92d1d8bb1833f18 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712393 |
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