مورد إلكتروني
Removing celiac disease-related gluten proteins from bread wheat while retaining technological roperties: a study with Chinese Spring deletion Lines
| العنوان: | Removing celiac disease-related gluten proteins from bread wheat while retaining technological roperties: a study with Chinese Spring deletion Lines |
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| المصدر: | ISSN: 1471-2229 |
| بيانات النشر: | 2009 |
| تفاصيل مُضافة: | van den Broeck, H.C. van Herpen, T.W.J.M. Schuit, C. Salentijn, E.M.J. |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Background - Gluten proteins can induce celiac disease (CD) in genetically susceptible individuals. In CD patients gluten-derived peptides are presented to the immune system, which leads to a CD4+ T-cell mediated immune response and inflammation of the small intestine. However, not all gluten proteins contain T-cell stimulatory epitopes. Gluten proteins are encoded by multigene loci present on chromosomes 1 and 6 of the three different genomes of hexaploid bread wheat (Triticum aestivum) (AABBDD). Results - The effects of deleting individual gluten loci on both the level of T-cell stimulatory epitopes in the gluten proteome and the technological properties of the flour were analyzed using a set of deletion lines of Triticum aestivum cv. Chinese Spring. The reduction of T-cell stimulatory epitopes was analyzed using monoclonal antibodies that recognize T-cell epitopes present in gluten proteins. The deletion lines were technologically tested with respect to dough mixing properties and dough rheology. The results show that removing the ¿-gliadin locus from the short arm of chromosome 6 of the D-genome (6DS) resulted in a significant decrease in the presence of T-cell stimulatory epitopes but also in a significant loss of technological properties. However, removing the ¿-gliadin, ¿-gliadin, and LMW-GS loci from the short arm of chromosome 1 of the D-genome (1DS) removed T-cell stimulatory epitopes from the proteome while maintaining technological properties. Conclusion - The consequences of these data are discussed with regard to reducing the load of T-cell stimulatory epitopes in wheat, and to contributing to the design of CD-safe wheat varieties |
| مصطلحات الفهرس: | alpha-gliadin, autoimmune disorders, biochemical basis, flour properties, gamma-gliadin, molecular-weight subunits, omega-gliadin, polymeric protein, storage proteins, triticum-aestivum, info:eu-repo/semantics/article, Article/Letter to editor, info:eu-repo/semantics/publishedVersion |
| URL: | |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess Wageningen University & Research |
| ملاحظة: | application/pdf BMC Plant Biology 9 (2009) ISSN: 1471-2229 ISSN: 1471-2229 English |
| أرقام أخرى: | NLWUP oai:library.wur.nl:wurpubs/378957 https://research.wur.nl/en/publications/removing-celiac-disease-related-gluten-proteins-from-bread-wheatTest- 10.1186/1471-2229-9-41 1200339530 |
| المصدر المساهم: | WUR STAFF PUBNS From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1200339530 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |