Notch1 signaling induces epithelial-mesenchymal transition in lens epithelium cells during hypoxia
| العنوان: | Notch1 signaling induces epithelial-mesenchymal transition in lens epithelium cells during hypoxia |
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| المؤلفون: | Wei Xiao, Lei Liu |
| المصدر: | BMC Ophthalmology, Vol 17, Iss 1, Pp 1-8 (2017) BMC Ophthalmology |
| بيانات النشر: | BMC, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Hypoxia-inducible factor-1 alpha (HIF-1α), Pathology, medicine.medical_specialty, Epithelial-Mesenchymal Transition, Blotting, Western, Lens Capsule, Crystalline, Motility, Cell junction, 03 medical and health sciences, Cobalt chloride (CoCl2), 0302 clinical medicine, Gentamicin protection assay, lcsh:Ophthalmology, Cell Movement, Cell polarity, medicine, Humans, Epithelial–mesenchymal transition, Receptor, Notch1, Cells, Cultured, Cell Proliferation, Notch1, biology, business.industry, Posterior capsular opacification (PCO), Epithelial Cells, General Medicine, Transfection, Capsule Opacification, Hypoxia (medical), Immunohistochemistry, Cell biology, Fibronectin, Epithelial-mesenchymal transition (EMT), Ophthalmology, 030104 developmental biology, Gene Expression Regulation, lcsh:RE1-994, 030220 oncology & carcinogenesis, embryonic structures, biology.protein, cardiovascular system, RNA, sense organs, medicine.symptom, business, Signal Transduction, Research Article |
| الوصف: | Background Posterior Capsular Opacification (PCO) is one of the most common complications of cataract surgery which can result in severe visual damage. Epithelial-Mesenchymal Transition (EMT) of lens epithelium cells (LEC) is the pathological basis of PCO. Recent research showed that hypoxia acted as an inducer of EMT through a Notch1/Snail1/E-cadherin pathway. However, it remains unclear whether the Notch1/Snail1/E-cadherin pathway is involved in PCO under hypoxia. Methods The morphology of SRA01/04 cells treating with Cobalt Chloride (CoCl2) was observed and the markers of EMT and Notch1/Snail1/E-cadherin pathway were analyzed by Western blot and Immunocytochemistry assay. Transwell invasion assay and Wound healing assay were used to detected the effect of p3 × FLAG-CMV-7-NICD1 transfection on the SRA01/04 cells. Results The SRA01/04 cells lost cell polarity and cell junction culturing with CoCl2. The expression of Keratin, Hypoxia-inducible factor-1 alpha (HIF-1α), Notch1, Snail1were upregulated, on the other side, Fibronectin and E-cadherin were downregulated in hypoxia. Furthermore, the overexpression of Notch1 induced the expression of E-cadherin and increased the invasion and migration ability of SRA01/04 cells. Conclusions These results suggest that Notch1/Snail1/E-cadherin pathway facilitates the EMT through HIF-1α in SRA01/04 cells during hypoxia and promotes LEC motility. |
| اللغة: | English |
| تدمد: | 1471-2415 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8304404cc81ad531030b3c1f73f80567Test http://link.springer.com/article/10.1186/s12886-017-0532-1Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8304404cc81ad531030b3c1f73f80567 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712415 |
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