Conditional deletion of E11/podoplanin in bone protects against load-induced osteoarthritis
| العنوان: | Conditional deletion of E11/podoplanin in bone protects against load-induced osteoarthritis |
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| المؤلفون: | Ekele Ikpegbu, Anish K. Amin, Behzad Javaheri, Scott Dillon, Andrew A. Pitsillides, Anna E. Törnqvist, Katherine Staines, Dylan N. Clements, David J. Buttle, Colin Farquharson |
| المصدر: | BMC Musculoskeletal Disorders BMC Musculoskeletal Disorders, Vol 20, Iss 1, Pp 1-11 (2019) Staines, K, Ikpegbu, E, Tornqvist, A, Dillon, S, Javaheri, B, Amin, A, Clements, D, Buttle, D, Pitsillides, A A & Farquharson, C 2019, ' Conditional deletion of E11/podoplanin in bone protects against load-induced osteoarthritis ', BMC Musculoskeletal Disorders, vol. 20, 344 . https://doi.org/10.1186/s12891-019-2731-9Test |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Biomedical Science Research Group, Cartilage, Articular, Male, osteocytes, subchondral bone, osteoarthritis, E11/podoplanin, Pathology, lcsh:Diseases of the musculoskeletal system, subchondral bone, Osteoarthritis, E11/podoplanin, Menisci, Tibial, Bortezomib, Weight-Bearing, Pathogenesis, Mice, 0302 clinical medicine, E11, Orthopedics and Sports Medicine, Mice, Knockout, 030222 orthopedics, Membrane Glycoproteins, Osteophyte, Subchondral bone, medicine.anatomical_structure, Health, Osteocyte, 616.7 Diseases of musculoskeletal system, RC Internal medicine, Medial meniscus, osteocytes, Research Article, medicine.drug, medicine.medical_specialty, Osteocytes, 03 medical and health sciences, Dogs, Rheumatology, Internal medicine, medicine, Animals, Humans, 030203 arthritis & rheumatology, business.industry, medicine.disease, Ageing, podoplanin, osteoarthritis, Disease Models, Animal, Podoplanin, Proteasome inhibitor, lcsh:RC925-935, business |
| الوصف: | Background Subchondral bone (SCB) thickening is one of the earliest detectable changes in osteoarthritic joints and is considered a potential trigger for subsequent articular cartilage degeneration. In this manuscript, we examine whether disruption to the SCB osteocyte network contributes to the initiation and pathogenesis of osteoarthritis. Methods We examined expression patterns of the glycoprotein E11/podoplanin by immunohistochemical labelling in murine, human and canine osteoarthritis models. We also examined the effects of twice-weekly administration of Bortezomib, a proteasome inhibitor which stabilises osteocyte E11 levels, to C57/BL6 wild-type male mice (1 mg/kg/day) for 8 weeks after surgical destabilisation of the medial meniscus. By inducing osteoarthritis-like changes in the right knee joint of 12-week-old male E11 hypomorphic mice (and corresponding controls) using a post-traumatic joint loading model, we also investigated whether a bone-specific E11 deletion in mice increases joint vulnerability to osteoarthritis. Articular cartilage degradation and osteophyte formation were assessed by histology and in line with the OARSI grading system. Results Our studies reveal increased E11 expression in osteocytes of human and canine osteoarthritic SCB. We found that Bortezomib administration had no effect on surgically-induced osteoarthritis, potentially due to a lack of the expected stabilisation of E11 in the SCB. We also found, in concordance with our previous work, wild-type mice exhibited significant load-induced articular cartilage lesions on the lateral femoral condyle (p |
| وصف الملف: | application/pdf; Word |
| تدمد: | 1471-2474 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e370547a177ef09ce25a75a7f5e4596aTest https://doi.org/10.1186/s12891-019-2731-9Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e370547a177ef09ce25a75a7f5e4596a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712474 |
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