Triptolide ameliorates fine particulate matter-induced podocytes injury via regulating NF-κB signaling pathway
| العنوان: | Triptolide ameliorates fine particulate matter-induced podocytes injury via regulating NF-κB signaling pathway |
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| المؤلفون: | Qiang Wan, Yanwei Liu, Zhongyong Liu, Ming Yang, Nana Tang, Peng Deng |
| المصدر: | BMC Molecular and Cell Biology BMC Molecular and Cell Biology, Vol 21, Iss 1, Pp 1-10 (2020) |
| بيانات النشر: | BioMed Central, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Fine particulate matter, 030232 urology & nephrology, Apoptosis, chemistry.chemical_compound, Mice, 0302 clinical medicine, Cell Movement, Malondialdehyde, Cytoskeleton, bcl-2-Associated X Protein, 0303 health sciences, biology, medicine.diagnostic_test, Chemistry, lcsh:Cytology, Podocytes, Intracellular Signaling Peptides and Proteins, NF-kappa B, Cell biology, Proto-Oncogene Proteins c-bcl-2, Signal transduction, Diterpenes, Nuclear factor kappa-B, Research Article, Signal Transduction, Cell Survival, Tripterygium, complex mixtures, Flow cytometry, Cell Line, Nephrin, Superoxide dismutase, 03 medical and health sciences, medicine, Animals, lcsh:QH573-671, Molecular Biology, 030304 developmental biology, Triptolide, L-Lactate Dehydrogenase, Superoxide Dismutase, Membrane Proteins, Cell Biology, Phenanthrenes, biology.protein, Podocin, Epoxy Compounds, Particulate Matter, Drugs, Chinese Herbal |
| الوصف: | Background PM2.5 is associated closely with an increased risk of membranous nephropathy (MN), however, whether PM2.5 could induce podocytes injury, the underlying pathology for MN, has not be thoroughly studied. Triptolide, an active component in Tripterygium wilfordii Hook F, is frequently used to treat MN in China, but its effects on PM2.5-induced podocytes injury is still largely unknown. Therefore, we evaluated the effects of PM2.5 on podocytes, and explored whether triptolide could improve PM2.5-induced podocytes injury and the possible underlying mechanisms. Results Podocytes were incubated with PM2.5 after being pre-treated with triptolide, viability, apoptosis rate and migratory capacity of podocytes were determined by CCK-8 assay, flow cytometry and Transwell assay, respectively. Additionally, the levels of lactate dehydrogenase (LDH), malondialdehyde (MDA), and superoxide dismutase (SOD) in podocytes, the cytoskeleton of podocytes, the protein expressions of nephrin, podocin, Bcl-2, Bax, nuclear factor kappa-B/p65 (NF-κB/p65) and phospho-inhibitor of NF-κB (p-IκBα) were measured. Our data showed that PM2.5 treatment significantly increased the disorganization of F-actin stress fibers, the damaged structural integrity of nucleus, the deranged and dissociated cytoskeleton in podocytes, increased the podocytes apoptosis rate, the levels of MDA and LDH, markedly up-regulated the protein expression of Bax, NF-κB/p65 and p-IκBα, down-regulated the protein expression of nephrin, podocin and Bcl-2, and significantly decreased the level of SOD, the migration rate and the viability of podocytes, compared with those of the untreated podocytes. These effects of PM2.5 on podocytes, however, were reversed by triptolide administration. Conclusion These results suggest that triptolide could prevent against PM2.5-induced podocytes injury via suppressing NF-κB signaling pathway. |
| اللغة: | English |
| تدمد: | 2661-8850 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93b7807d5ae3a4714cbe796698acfdbbTest http://europepmc.org/articles/PMC6998203Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....93b7807d5ae3a4714cbe796698acfdbb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 26618850 |
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