Analysis of HIV-1 drug resistant mutations by line probe assay and direct sequencing in a cohort of therapy naive HIV-1 infected Italian patients
| العنوان: | Analysis of HIV-1 drug resistant mutations by line probe assay and direct sequencing in a cohort of therapy naive HIV-1 infected Italian patients |
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| المؤلفون: | C Re, M, Monari, P, Bon, I, Gibellini, D, Vitone, F, Borderi, M, M La Placa |
| المصدر: | BMC Microbiology BMC Microbiology, Vol 1, Iss 1, p 30 (2001) |
| بيانات النشر: | BioMed Central, 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | drug resistant, Molecular Sequence Data, lcsh:QR1-502, HIV Infections, HIV Protease Inhibitors, Microbial Sensitivity Tests, Viral Load, mutations, HIV-1, drug resistant, mutations, lcsh:Microbiology, Cohort Studies, Amino Acid Substitution, Italy, Drug Resistance, Viral, HIV-1, Reverse Transcriptase Inhibitors, Research Article |
| الوصف: | Background The routine determination of drug resistance in newly HIV-1 infected individuals documents a potential increase in the transmission of drug-resistant variants. Plasma samples from twenty seven therapy naive HIV-1 infected Italian patients were analyzed by the line probe assay (LIPA) and the TruGene HIV-1 assay for the detection of mutations conferring resistance to HIV-1. Results Both tests disclosed amino-acid substitutions associated with resistance in a variable number of patients. In particular, two mutations (K70R and V118I), detectable by LIPA and by sequencing analysis respectively, revealed resistance to NRTIs in two plasma samples. At least three mutations conferring resistance to NNRTIs, not detectable by commercial LIPA, able to reveal mutations associated only with nucleoside reverse transcriptase analogues, were disclosed by viral sequence analysis. Moreover, most samples showed mutations correlated with resistance to protease inhibitors. Remarkably, a key mutation, like V82A (found as a mixture), and some "indeterminate" results (9 samples), due the absence of signal on the lines corresponding to a specific probe, was revealed only by LIPA, while a variable number of secondary mutations was detectable only by TruGene HIV-1 assay. Conclusion Even if further studies are necessary to establish the impact of different tests on the evaluation of drug-resistant strains transmission, LIPA might be useful in a wide population analysis, where bulk results are needed in a short time, while sequencing analysis, able to detect mutations conferring resistance to both NRTIs and NNRTIs, might be considered a more complete assay, albeit more expensive and more technically complex. |
| اللغة: | English |
| تدمد: | 1471-2180 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::a5b905d01713c80db00a5a71f9f0fe23Test http://europepmc.org/articles/PMC60646Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.pmid.dedup....a5b905d01713c80db00a5a71f9f0fe23 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712180 |
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