التفاصيل البيبلوغرافية
| العنوان: |
Evaluation of saliva self-collection devices for SARS-CoV-2 diagnostics. |
| المؤلفون: |
Allicock, Orchid M., Petrone, Mary E., Yolda-Carr, Devyn, Breban, Mallery, Walsh, Hannah, Watkins, Anne E., Rothman, Jessica E., Farhadian, Shelli F., Grubaugh, Nathan D., Wyllie, Anne L. |
| المصدر: |
BMC Infectious Diseases; 3/25/2022, Vol. 22 Issue 1, p1-9, 9p |
| مصطلحات موضوعية: |
SALIVA, SUPPLY chain disruptions, SARS-CoV-2, MEDICAL assistance, SUMMER |
| مستخلص: |
Background: There is an urgent need to expand testing for SARS-CoV-2 and other respiratory pathogens as the global community struggles to control the COVID-19 pandemic. Current diagnostic methods can be affected by supply chain bottlenecks and require the assistance of medical professionals, impeding the implementation of large-scale testing. Self-collection of saliva may solve these problems, as it can be completed without specialized training and uses generic materials.Methods: We observed 30 individuals who self-collected saliva using four different collection devices and analyzed their feedback. Two of these devices, a funnel and bulb pipette, were used to evaluate at-home saliva collection by 60 individuals. SARS-CoV-2-spiked saliva samples were subjected to temperature cycles designed to simulate the conditions the samples might be exposed to during the summer and winter seasons and sensitivity of detection was evaluated.Results: All devices enabled the safe, unsupervised self-collection of saliva. The quantity and quality of the samples received were acceptable for SARS-CoV-2 diagnostic testing, as determined by human RNase P detection. There was no significant difference in SARS-CoV-2 nucleocapsid gene (N1) detection between the freshly spiked samples and those incubated with the summer and winter profiles.Conclusion: We demonstrate inexpensive, generic, buffer free collection devices suitable for unsupervised and home saliva self-collection. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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