دورية أكاديمية
Distinct and overlapping gene regulatory networks in BMP- and HDAC-controlled cell fate determination in the embryonic forebrain
| العنوان: | Distinct and overlapping gene regulatory networks in BMP- and HDAC-controlled cell fate determination in the embryonic forebrain |
|---|---|
| المؤلفون: | Scholl Catharina, Weiβmüller Kathrin, Holenya Pavlo, Shaked-Rabi Maya, Tucker Kerry L, Wölfl Stefan |
| المصدر: | BMC Genomics, Vol 13, Iss 1, p 298 (2012) |
| بيانات النشر: | BMC, 2012. |
| سنة النشر: | 2012 |
| المجموعة: | LCC:Biotechnology LCC:Genetics |
| مصطلحات موضوعية: | Biotechnology, TP248.13-248.65, Genetics, QH426-470 |
| الوصف: | Abstract Background Both bone morphogenetic proteins (BMPs) and histone deacetylases (HDACs) have previously been established to play a role in the development of the three major cell types of the central nervous system: neurons, astrocytes, and oligodendrocytes. We have previously established a connection between these two protein families, showing that HDACs suppress BMP-promoted astrogliogenesis in the embryonic striatum. Since HDACs act in the nucleus to effect changes in transcription, an unbiased analysis of their transcriptional targets could shed light on their downstream effects on BMP-signaling. Results Using neurospheres from the embryonic striatum as an in vitro system to analyze this phenomenon, we have performed microarray expression profiling on BMP2- and TSA-treated cultures, followed by validation of the findings with quantitative RT-PCR and protein analysis. In BMP-treated cultures we first observed an upregulation of genes involved in cell-cell communication and developmental processes such as members of BMP and canonical Wnt signaling pathways. In contrast, in TSA-treated cultures we first observed an upregulation of genes involved in chromatin modification and transcription. Interestingly, we could not record direct changes in the protein levels of canonical members of BMP2 signaling, but we did observe an upregulation of both the transcription factor STAT3 and its active isoform phospho-STAT3 at the protein level. Conclusions STAT3 and SMAD1/5/8 interact synergistically to promote astrogliogenesis, and thus we show for the first time that HDACs act to suppress BMP-promoted astrogliogenesis by suppression of the crucial partner STAT3. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1471-2164 10305254 |
| العلاقة: | http://www.biomedcentral.com/1471-2164/13/298Test; https://doaj.org/toc/1471-2164Test |
| DOI: | 10.1186/1471-2164-13-298 |
| الوصول الحر: | https://doaj.org/article/20c10305254846d3ae6f3845091449f9Test |
| رقم الانضمام: | edsdoj.20c10305254846d3ae6f3845091449f9 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14712164 10305254 |
|---|---|
| DOI: | 10.1186/1471-2164-13-298 |