Bacillus thuringiensis Cry1Ca-resistant Spodoptera exigua lacks expression of one of four Aminopeptidase N genes
| العنوان: | Bacillus thuringiensis Cry1Ca-resistant Spodoptera exigua lacks expression of one of four Aminopeptidase N genes |
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| المؤلفون: | William J. Moar, Tsanko Gechev, Salvador Herrero, Petra L. Bakker, Ruud A. de Maagd |
| المصدر: | BMC Genomics BMC Genomics, Vol 6, Iss 1, p 96 (2005) BMC Genomics 6 (2005) BMC Genomics, 6, 96-96 |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Gene Expression, bombyx-mori, brush-border membrane, Hemolysin Proteins, manduca-sexta, Bacillus thuringiensis, Cluster Analysis, Cloning, Molecular, heliothis-virescens, Phylogeny, biology, Nucleic Acid Hybridization, plutella-xylostella, cry1a toxins, Up-Regulation, PRI Bioscience, toxin receptor, hormones, hormone substitutes, and hormone antagonists, Biotechnology, Delta endotoxin, Research Article, animal structures, DNA, Complementary, lcsh:QH426-470, lcsh:Biotechnology, Bacterial Toxins, Molecular Sequence Data, Down-Regulation, midgut, Spodoptera, CD13 Antigens, Bacterial Proteins, Complementary DNA, lcsh:TP248.13-248.65, Exigua, Genetics, Escherichia coli, Animals, Amino Acid Sequence, RNA, Messenger, Pest Control, Biological, DNA Primers, Gene Library, Bacillus thuringiensis Toxins, Models, Genetic, Sequence Homology, Amino Acid, cDNA library, molecular-cloning, fungi, Midgut, Sequence Analysis, DNA, biology.organism_classification, Blotting, Northern, Molecular biology, Endotoxins, lcsh:Genetics, Gene Expression Regulation, Suppression subtractive hybridization, delta-endotoxin, Software |
| الوصف: | Background Insecticidal toxins from Bacillus thuringiensis bind to receptors on midgut epithelial cells of susceptible insect larvae. Aminopeptidases N (APNs) from several insect species have been shown to be putative receptors for these toxins. Here we report the cloning and expression analysis of four APN cDNAs from Spodoptera exigua. Results Suppression Subtractive Hybridization (SSH) was used to construct cDNA libraries of genes that are up-and down-regulated in the midgut of last instar larvae of beet armyworm, S. exigua exposed to B. thuringiensis Cry1Ca toxin. Among the clones from the SSH libraries, cDNA fragments coding for two different APNs were obtained (APN2 and APN4). A similar procedure was employed to compare mRNA differences between susceptible and Cry1Ca resistant S. exigua. Among the clones from this last comparison, cDNA fragments belonging to a third APN (APN1) were detected. Using sequences obtained from the three APN cDNA fragments and degenerate primers for a fourth APN (APN3), the full length sequences of four S. exigua APN cDNAs were obtained. Northern blot analysis of expression of the four APNs showed complete absence of APN1 expression in the resistant insects, while the other three APNs showed similar expression levels in the resistant and susceptible insects. Conclusion We have cloned and characterized four different midgut APN cDNAs from S. exigua. Expression analysis revealed the lack of expression of one of these APNs in the larvae of a Cry1Ca-resistant colony. Combined with previous evidence that shows the importance of APN in the mode of action of B. thuringiensis toxins, these results suggest that the lack of APN1 expression plays a role in the resistance to Cry1Ca in this S. exigua colony. |
| وصف الملف: | application/pdf; application/octet-stream |
| تدمد: | 1471-2164 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::98900af212e63c737041a99dd177843dTest https://pubmed.ncbi.nlm.nih.gov/15978131Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....98900af212e63c737041a99dd177843d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712164 |
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