Comparative transcriptomic analysis of follicle-enclosed oocyte maturational and developmental competence acquisition in two non-mammalian vertebrates
| العنوان: | Comparative transcriptomic analysis of follicle-enclosed oocyte maturational and developmental competence acquisition in two non-mammalian vertebrates |
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| المؤلفون: | Julien Bobe, Franck Chesnel, Maella Gohin |
| المساهمون: | Institut de Génétique et Développement de Rennes (IGDR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-IFR140-Centre National de la Recherche Scientifique (CNRS), Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA)-IFR140, ANR-08-GENM-033 (OSCILE),ANR-08-GENM-033 (OSCILE), Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Institut National de la Recherche Agronomique (INRA), ANR-08-GENM-0033,OSCILE,Interactions ovocytes-cellules somatiques, une étude de génomique comparative chez les vertébrés(2008), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), De Villemeur, Hervé, Génomique - Interactions ovocytes-cellules somatiques, une étude de génomique comparative chez les vertébrés - - OSCILE2008 - ANR-08-GENM-0033 - GENM - VALID |
| المصدر: | BMC Genomics BMC Genomics (11:18), 1-19. (2010) BMC Genomics, Vol 11, Iss 1, p 18 (2010) BMC Genomics, BioMed Central, 2010, 11 (1), pp.18. ⟨10.1186/1471-2164-11-18⟩ BMC Genomics, 2010, 11 (1), pp.18. ⟨10.1186/1471-2164-11-18⟩ |
| بيانات النشر: | Springer Nature |
| مصطلحات موضوعية: | Candidate gene, MESH: Sequence Analysis, DNA, Xenopus, fish, salmonid, MESH: Amino Acid Sequence, maturation ovocytaire, Oogenesis, Polymerase Chain Reaction, follicule ovarien, Transcriptome, Xenopus laevis, 0302 clinical medicine, poisson, MESH: Gene Expression Regulation, Developmental, Biologie de la reproduction, Data Mining, MESH: Animals, [SDV.BDD]Life Sciences [q-bio]/Development Biology, Oligonucleotide Array Sequence Analysis, Genetics, Animal biology, salmonidae, 0303 health sciences, Reproductive Biology, Comparative Genomic Hybridization, biology, mammifère, Gene Expression Regulation, Developmental, ovocyte, MESH: Oogenesis, ovogenèse, Trout, MESH: Oncorhynchus mykiss, Oncorhynchus mykiss, Female, expression des gènes, Research Article, Biotechnology, MESH: Endoribonucleases, endocrine system, animal structures, lcsh:QH426-470, lcsh:Biotechnology, Molecular Sequence Data, MESH: Sequence Alignment, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, MESH: Oocytes, reproduction, 03 medical and health sciences, MESH: Gene Expression Profiling, pcr, MESH: Xenopus laevis, lcsh:TP248.13-248.65, [SDV.BDD] Life Sciences [q-bio]/Development Biology, Biologie animale, Endoribonucleases, Animals, Amino Acid Sequence, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, Gene, [SDV.BDLR] Life Sciences [q-bio]/Reproductive Biology, 030304 developmental biology, MESH: Molecular Sequence Data, ovogénèse, urogenital system, MESH: Data Mining, amphibien, Gene Expression Profiling, MESH: Polymerase Chain Reaction, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, Sequence Analysis, DNA, biology.organism_classification, vertébré, Gene expression profiling, MESH: Comparative Genomic Hybridization, lcsh:Genetics, xenope, MESH: Oligonucleotide Array Sequence Analysis, Oocytes, Rainbow trout, MESH: Female, Sequence Alignment, 030217 neurology & neurosurgery, truite arc en ciel |
| الوصف: | Background In vertebrates, late oogenesis is a key period during which the oocyte acquires its ability to resume meiosis (i.e. maturational competence) and to develop, once fertilized, into a normal embryo (i.e. developmental competence). However, the molecular mechanisms involved in these key biological processes are far from being fully understood. In order to identify key mechanisms conserved among teleosts and amphibians, we performed a comparative analysis using ovarian tissue sampled at successive steps of the maturational competence acquisition process in the rainbow trout (Oncorhynchus mykiss) and in the clawed toad (Xenopus laevis). Our study aimed at identifying common differentially expressed genes during late oogenesis in both species. Using an existing transcriptomic analysis that had previously been carried out in rainbow trout, candidate genes were selected for subsequent quantitative PCR-based comparative analysis. Results Among the 1200 differentially expressed clones in rainbow trout, twenty-six candidate genes were selected for further analysis by real-time PCR in both species during late oogenesis. Among these genes, eight had similar expression profiles in trout and Xenopus. Six genes were down-regulated during oocyte maturation (cyp19a1, cyp17a1, tescalcin, tfr1, cmah, hsd11b3) while two genes exhibited an opposite pattern (apoc1, star). In order to document possibly conserved molecular mechanisms, four genes (star, cyp19a1, cyp17a1 and hsd11b3) were further studied due to their known or suspected role in steroidogenesis after characterization of the orthology relationships between rainbow trout and Xenopus genes. Apoc1 was also selected for further analysis because of its reported function in cholesterol transport, which may modulate steroidogenesis by regulating cholesterol bioavailability in the steroidogenic cells. Conclusions We have successfully identified orthologous genes exhibiting conserved expression profiles in the ovarian follicle during late oogenesis in both trout and Xenopus. While some identified genes were previously uncharacterized during Xenopus late oogenesis, the nature of these genes has pointed out molecular mechanisms possibly conserved in amphibians and teleosts. It should also be stressed that in addition to the already suspected importance of steroidogenesis in maturational competence acquisition, our approach has shed light on other regulatory pathways which may be involved in maturational and developmental competence acquisitions that will require further studies. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1471-2164 |
| DOI: | 10.1186/1471-2164-11-18 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::48429b2e7a394cbb225869d25eeffd1dTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....48429b2e7a394cbb225869d25eeffd1d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712164 |
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| DOI: | 10.1186/1471-2164-11-18 |