Progeny-testing of full-sibs IBD in a SSC2 QTL region highlights epistatic interactions for fatness traits in pigs
| العنوان: | Progeny-testing of full-sibs IBD in a SSC2 QTL region highlights epistatic interactions for fatness traits in pigs |
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| المؤلفون: | Yvon Billon, Nathalie Iannuccelli, Flavie Tortereau, Denis Milan, Marie Pierre Sanchez, Jean Pierre Bidanel, Juliette Riquet, Katia Feve, Hélène Gilbert |
| المساهمون: | Laboratoire de Génétique Cellulaire (LGC), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Génétique Expérimentale en Productions Animales (GEPA), Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Tortereau, Flavie |
| المصدر: | BMC Genetics BMC Genetics, BioMed Central, 2011, 12, online (october), Non paginé. ⟨10.1186/1471-2156-12-92⟩ BMC Genetics october (12, online), Non paginé. (2011) BMC Genetics, Vol 12, Iss 1, p 92 (2011) BMC Genetics, 12 BMC Genetics 12 (2011) |
| بيانات النشر: | Springer Nature |
| مصطلحات موضوعية: | Male, pig, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, Progeny testing, Swine, large white-pigs, Breeding, Family-based QTL mapping, Inbreeding, Genetics(clinical), Genetics (clinical), Genetics, 0303 health sciences, igf2 locus, qtl, Chromosome Mapping, food and beverages, 04 agricultural and veterinary sciences, Pedigree, Phenotype, muscle mass, Adipose Tissue, epistatic interaction, Female, imprinted qtl, Research Article, Genetic Markers, Meat, lcsh:QH426-470, Fatty Acid Elongases, growth, Quantitative Trait Loci, sus-scrofa, Locus (genetics), genome scan, Animal Breeding and Genomics, Quantitative trait locus, Biology, Polymorphism, Single Nucleotide, 03 medical and health sciences, Quantitative Trait, Heritable, Acetyltransferases, Insulin-Like Growth Factor II, Animals, Fokkerij en Genomica, mutation, Allele, 030304 developmental biology, Haplotype, 0402 animal and dairy science, 040201 dairy & animal science, alternative models, lcsh:Genetics, backfat thickness, Haplotypes, Backcrossing, WIAS, Epistasis, carcass, Microsatellite Repeats |
| الوصف: | Background Many QTL have been detected in pigs, but very few of them have been fine-mapped up to the causal mutation. On SSC2, the IGF2-intron3-G3072A mutation has been described as the causative polymorphism for a QTL underlying muscle mass and backfat deposition, but further studies have demonstrated that at least one additional QTL should segregate downstream of this mutation. A marker-assisted backcrossing design was set up in order to confirm the segregation of this second locus, reduce its confidence interval and better understand its mode of segregation. Results Five recombinant full-sibs, with genotype G/G at the IGF2 mutation, were progeny-tested. Only two of them displayed significant QTL for fatness traits although four inherited the same paternal and maternal chromosomes, thus exhibiting the same haplotypic contrast in the QTL region. The hypothesis of an interaction with another region in the genome was proposed to explain these discrepancies and after a genome scan, four different regions were retained as potential interacting regions with the SSC2 QTL. A candidate interacting region on SSC13 was confirmed by the analysis of an F2 pedigree, and in the backcross pedigree one haplotype in this region was found to mask the SSC2 QTL effect. Conclusions Assuming the hypothesis of interactions with other chromosomal regions, the QTL could be unambiguously mapped to a 30 cM region delimited by recombination points. The marker-assisted backcrossing design was successfully used to confirm the segregation of a QTL on SSC2 and, because full-sibs that inherited the same alleles from their two parents were analysed, the detection of epistatic interactions could be performed between alleles and not between breeds as usually done with the traditional Line-Cross model. Additional analyses of other recombinant sires should provide more information to further improve the fine-mapping of this locus, and confirm or deny the interaction identified between chromosomes 2 and 13. |
| وصف الملف: | application/pdf; application/octet-stream |
| اللغة: | English |
| تدمد: | 1471-2156 |
| DOI: | 10.1186/1471-2156-12-92 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::facbfde0459747ac8ddbedae8b113befTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....facbfde0459747ac8ddbedae8b113bef |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712156 |
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| DOI: | 10.1186/1471-2156-12-92 |