Lactobacillus casei DG and its postbiotic reduce the inflammatory mucosal response: an ex-vivo organ culture model of post-infectious irritable bowel syndrome
| العنوان: | Lactobacillus casei DG and its postbiotic reduce the inflammatory mucosal response: an ex-vivo organ culture model of post-infectious irritable bowel syndrome |
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| المؤلفون: | C. Sgamato, Alba Rocco, Umberto Salvatore, Gerardo Nardone, D. Angrisani, Barbara Iovine, Pietro Coccoli, Debora Compare |
| المساهمون: | Compare, Debora, Rocco, Alba, Coccoli, Pietro, Angrisani, Debora, Sgamato, Costantino, Iovine, Barbara, Salvatore, Umberto, Nardone, Gerardo |
| المصدر: | BMC Gastroenterology BMC Gastroenterology, Vol 17, Iss 1, Pp 1-8 (2017) |
| بيانات النشر: | Springer Science and Business Media LLC, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Lipopolysaccharides, Male, 0301 basic medicine, Postbiotic, Gut flora, Probiotic, Irritable Bowel Syndrome, 0302 clinical medicine, Intestinal mucosa, Interleukin-1alpha, Lactobacillus, Medicine, Intestinal Mucosa, Post-infectious irritable bowel syndrome, biology, Gastroenterology, Interleukin, General Medicine, Middle Aged, Interleukin-10, Lacticaseibacillus casei, Interleukin 10, Female, 030211 gastroenterology & hepatology, Case-Control Studie, Lactobacillus casei, Research Article, Human, Colon, Blotting, Western, Lipopolysaccharide, In Vitro Techniques, Real-Time Polymerase Chain Reaction, Organ culture, 03 medical and health sciences, Organ Culture Techniques, Ileum, Humans, RNA, Messenger, lcsh:RC799-869, Inflammation, In Vitro Technique, Interleukin-6, business.industry, Probiotics, Interleukin-8, biology.organism_classification, Gastrointestinal Microbiome, Toll-Like Receptor 4, 030104 developmental biology, Case-Control Studies, Immunology, Organ Culture Technique, lcsh:Diseases of the digestive system. Gastroenterology, business, Ex vivo |
| الوصف: | Background The evidence on the role of gut microbiota in post-infectious irritable bowel syndrome (PI-IBS) is convincing. Lactobacillus spp. positively affect IBS symptoms, although the mechanisms through which probiotics exert their beneficial effects are largely unknown. The aim of the study is to evaluate the role of Lactobacillus casei DG (LC-DG) and its postbiotic (PB) in modulating the inflammatory/immune-response in PI-IBS in an ex-vivo organ culture model. Methods Ex vivo cultures of ileal and colonic mucosa from 10 PI-IBS, diarrhea predominant subtype (D) patients, and 10 healthy controls (HC) were treated with LPS, LC-DG and PB. Interleukin (IL)-1α, IL-6, IL-8 and IL-10 mRNA levels were assessed by real-time PCR and Toll like receptor 4 (TLR-4) protein expression by Western blotting. Results At baseline, IL-1α, IL-6 and IL-8 mRNA levels as well as TLR-4 protein expression were significantly higher while IL-10 mRNA levels were lower in PI-IBS D than in HC in both ileum and colon. LC-DG and PB significantly reduced the mRNA levels of pro-inflammatory cytokines and TLR-4 while increased that of IL-10 after LPS stimulation. The protective effect was more pronounced for PB than LC-DG treatment. Conclusion LC-DG and its PB attenuate the inflammatory mucosal response in an ex-vivo organ culture model of PI-IBS D. |
| تدمد: | 1471-230X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c621c7de1810f02ff4ffb9c3de4a404aTest https://doi.org/10.1186/s12876-017-0605-xTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c621c7de1810f02ff4ffb9c3de4a404a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1471230X |
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