Age-related changes in rat bone-marrow mesenchymal stem cell plasticity
| العنوان: | Age-related changes in rat bone-marrow mesenchymal stem cell plasticity |
|---|---|
| المؤلفون: | Faizal Z. Asumda, P. Bryant Chase |
| المصدر: | BMC Cell Biology, Vol 12, Iss 1, p 44 (2011) BMC Cell Biology |
| بيانات النشر: | BMC, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Homeobox protein NANOG, Aging, medicine.medical_treatment, Clinical uses of mesenchymal stem cells, Bone Morphogenetic Protein 2, Bone Marrow Cells, Biology, Muscle Development, medicine, Animals, Insulin-Like Growth Factor I, lcsh:QH573-671, Cells, Cultured, Stem cell transplantation for articular cartilage repair, lcsh:Cytology, Growth factor, SOXB1 Transcription Factors, Mesenchymal stem cell, Age Factors, Nanog Homeobox Protein, Gene Expression Regulation, Developmental, Mesenchymal Stem Cells, Cell Biology, Stem-cell therapy, Cell biology, Rats, Adult Stem Cells, Connexin 43, Fibroblast Growth Factor 2, Octamer Transcription Factor-3, Biomarkers, Adult stem cell, Transcription Factors, Research Article |
| الوصف: | Background The efficacy of adult stem cells is known to be compromised as a function of age. This therefore raises questions about the effectiveness of autologous cell therapy in elderly patients. Results We demonstrated that the expression profile of stemness markers was altered in BM-MSCs derived from old rats. BM-MSCs from young rats (4 months) expressed Oct-4, Sox-2 and NANOG, but we failed to detect Sox-2 and NANOG in BM-MSCs from older animals (15 months). Chondrogenic, osteogenic and adipogenic potential is compromised in old BM-MSCs. Stimulation with a cocktail mixture of bone morphogenetic protein (BMP-2), fibroblast growth factor (FGF-2) and insulin-like growth factor (IGF-1) induced cardiomyogenesis in young BM-MSCs but not old BM-MSCs. Significant differences in the expression of gap junction protein connexin-43 were observed between young and old BM-MSCs. Young and old BM-MSCs fused with neonatal ventricular cardiomyocytes in co-culture and expressed key cardiac transcription factors and structural proteins. Cells from old animals expressed significantly lower levels of VEGF, IGF, EGF, and G-CSF. Significantly higher levels of DNA double strand break marker γ-H2AX and diminished levels of telomerase activity were observed in old BM-MSCs. Conclusion The results suggest age related differences in the differentiation capacity of BM-MSCs. These changes may affect the efficacy of BM-MSCs for use in stem cell therapy. |
| اللغة: | English |
| تدمد: | 1471-2121 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ff9bbbd0c85044347915b0ac14d4a28Test http://www.biomedcentral.com/1471-2121/12/44Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9ff9bbbd0c85044347915b0ac14d4a28 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712121 |
|---|