MiR-138-5p targets RUNX2 to inhibit osteogenic differentiation of aortic valve interstitial cells via Wnt/β-catenin signaling pathway
| العنوان: | MiR-138-5p targets RUNX2 to inhibit osteogenic differentiation of aortic valve interstitial cells via Wnt/β-catenin signaling pathway |
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| المؤلفون: | Fei Yan, Qiang Huo, Weimin Zhang, Tingting Wu, Daniyaer Dilimulati, Lin Shi |
| المصدر: | BMC Cardiovascular Disorders, Vol 22, Iss 1, Pp 1-8 (2022) BMC Cardiovascular Disorders |
| بيانات النشر: | BMC, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Adult, Male, Wnt/β-catenin, miR-138-5p, Research, RUNX2, Calcinosis, Cell Differentiation, Core Binding Factor Alpha 1 Subunit, Aortic Valve Stenosis, MicroRNAs, Gene Expression Regulation, Calcific aortic valve disease (CAVD), Osteogenesis, Aortic Valve, Osteogenic differentiation, RC666-701, Humans, Diseases of the circulatory (Cardiovascular) system, Female, Aortic valve interstitial cells (AVICs), Cardiology and Cardiovascular Medicine, Wnt Signaling Pathway, Cells, Cultured, beta Catenin |
| الوصف: | Background Human aortic valve interstitial cells (hAVICs) are a key factor in the pathogenesis of calcific aortic valve disease (CAVD). This research examines the role and mechanism of microRNA miR-138-5p in osteogenic differentiation of hAVICs. Methods RT-qPCR analysis was applied for detecting miR-138-5p and RUNX2 expression in valve tissues of CAVD patients and controls. On completion of induction of osteogenic differentiation of hAVICs, and after overexpression or interference of miR-138-5p expression, the condition of osteogenic differentiation and calcification of hAVICs was confirmed using alkaline phosphatase staining and alizarin red staining. Subsequently, western blot was utilized to detect the expression of osteogenesis-related proteins OPN and ALP, and Wnt/β-catenin signaling pathway-related proteins. Finally, the relationship between miR-138-5p and RUNX2 was validated by dual-luciferase reporter assay and Pearson’s correlation test. Results Down-regulation of miR-138-5p was found in CAVD patients and during osteogenic differentiation of hAVICs. Overexpression of miR-138-5p contribute to the inhibition of osteoblast differentiation and calcium deposition in hAVICs, and of ALP and OPN protein expression. RUNX2 was a target gene of miR-138-5p, and it was negatively correlated with miR-138-5p in CAVD. Additionally, overexpression of RUNX2 could reverse the inhibitory effect of miR-138-5p on osteogenic differentiation of hAVICs. Conclusion miR-138-5p can act as a positive regulator of osteogenic differentiation in CAVD patients to involve in inhibiting valve calcification, which is achieved through RUNX2 and Wnt/β-catenin signaling pathway. |
| اللغة: | English |
| تدمد: | 1471-2261 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33bd5933c6f99a56b73ca18104f8455aTest https://doaj.org/article/f0866dfb20a540fcaad44cebd50df8f1Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....33bd5933c6f99a56b73ca18104f8455a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712261 |
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