Correction to: Plexin-B1 silencing inhibits ovarian cancer cell migration and invasion
| العنوان: | Correction to: Plexin-B1 silencing inhibits ovarian cancer cell migration and invasion |
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| المؤلفون: | Yin Chen, Shixuan Wang, Jianfeng Zhou, Ting Zhou, Shuangmei Ye, Xing Hao, Li Ji, Yiqun Zhang, Ding Ma, Lanying You, Juncheng Wei, Li Zhou, Yongjun Wang, Mingfu Wu, Gang Xu, Xuefeng Jiang |
| المصدر: | BMC Cancer, Vol 19, Iss 1, Pp 1-2 (2019) BMC Cancer |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Adult, 0301 basic medicine, Cancer Research, Nerve Tissue Proteins, Receptors, Cell Surface, lcsh:RC254-282, 03 medical and health sciences, 0302 clinical medicine, Text mining, Cell Movement, Surgical oncology, Cell Line, Tumor, Genetics, Humans, Gene silencing, Medicine, Neoplasm Invasiveness, Gene Silencing, Aged, Cell Proliferation, Ovarian Neoplasms, business.industry, Correction, Cell migration, Middle Aged, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, medicine.disease, Gene Expression Regulation, Neoplastic, 030104 developmental biology, Oncology, Lymphatic Metastasis, 030220 oncology & carcinogenesis, Cancer research, Female, business, Ovarian cancer, Proto-Oncogene Proteins c-akt, Plexin b1 |
| الوصف: | Elevated Plexin-B1 expression has been found in diverse human cancers and in non-neoplastic tissues, and it mediates diverse biological and pathological activities. However, whether or not Plexin-B1 expression is involved in human ovarian tumors remains unclear. In the present study, Plexin-B1 expression was explored in benign and malignant human ovarian tumor tissues. In addition, the impact of Plexin-B1 expression on ovarian cancer cell proliferation, migration and invasion were investigated in vitro.Plexin-B1 expression was analyzed in normal and benign ovarian tissues and serous ovarian tumors (both borderline and malignant) by immunohistochemical staining, as well as in four human ovarian cancer cell lines (A2780, C13*, SKOV3, and OV2008) by RT-PCR and western blot analyses. Furthermore, endogenous Plexin-B1 expression was suppressed by Plexin-B1 siRNA in SKOV3 cells, which overexpress Plexin-B1. Protein levels of Plexin-B1, AKT and AKTSer473 were examined by western blot analysis. Cell proliferation, migration and invasion were measured with MTT, wound healing and boyden chamber assays, respectively, and the cytoskeleton was monitored via F-actin staining.Expression levels of Plexin-B1 protein were significantly higher in serous ovarian carcinomas than in normal ovaries or benign ovarian neoplasms, and in the former, Plexin-B1 expression was positively correlated with lymphatic metastasis, and the membrane and cytoplasm of cancer cells stained positively. SKOV3 cells displayed the highest Plexin-B1 expression at both the mRNA and protein levels among the four tested human ovarian cancer cell lines and was selected as a cell model for further in vitro experiments. Plexin-B1 siRNA significantly suppressed phosphorylation of AKT at Ser473 in SKOV3 cells, but it did not alter total AKT expression. In addition, silencing of Plexin-B1 in SKOV3 cells inhibited cell migration and invasion and reorganized the cytoskeleton, whereas cell proliferation was not affected.Plexin-B1 expression correlates with malignant phenotypes of serous ovarian tumors, probably via phosphorylation of AKT at Ser473, suggesting that Plexin-B1 might be a useful biomarker and/or a novel therapeutic target. |
| تدمد: | 1471-2407 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19472321277bf37fd5facde1f0ac9884Test https://doi.org/10.1186/s12885-019-6367-9Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....19472321277bf37fd5facde1f0ac9884 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712407 |
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