التفاصيل البيبلوغرافية
| العنوان: |
RNA-Seq in 296 phased trios provides a high-resolution map of genomic imprinting. |
| المؤلفون: |
Jadhav, Bharati, Monajemi, Ramin, Gagalova, Kristina K., Ho, Daniel, Draisma, Harmen H. M., van de Wiel, Mark A., Franke, Lude, Heijmans, Bastiaan T., van Meurs, Joyce, Jansen, Rick, 't Hoen, Peter A. C., Sharp, Andrew J., Kiełbasa, Szymon M. |
| المصدر: |
BMC Biology; 6/24/2019, Vol. 17 Issue 1, p1-20, 20p |
| مصطلحات موضوعية: |
GENOMIC imprinting, LYMPHOBLASTOID cell lines, GENETIC regulation, GENE expression |
| مصطلحات جغرافية: |
NETHERLANDS |
| مستخلص: |
Background: Identification of imprinted genes, demonstrating a consistent preference towards the paternal or maternal allelic expression, is important for the understanding of gene expression regulation during embryonic development and of the molecular basis of developmental disorders with a parent-of-origin effect. Combining allelic analysis of RNA-Seq data with phased genotypes in family trios provides a powerful method to detect parent-of-origin biases in gene expression. Results: We report findings in 296 family trios from two large studies: 165 lymphoblastoid cell lines from the 1000 Genomes Project and 131 blood samples from the Genome of the Netherlands (GoNL) participants. Based on parental haplotypes, we identified > 2.8 million transcribed heterozygous SNVs phased for parental origin and developed a robust statistical framework for measuring allelic expression. We identified a total of 45 imprinted genes and one imprinted unannotated transcript, including multiple imprinted transcripts showing incomplete parental expression bias that was located adjacent to strongly imprinted genes. For example, PXDC1, a gene which lies adjacent to the paternally expressed gene FAM50B, shows a 2:1 paternal expression bias. Other imprinted genes had promoter regions that coincide with sites of parentally biased DNA methylation identified in the blood from uniparental disomy (UPD) samples, thus providing independent validation of our results. Using the stranded nature of the RNA-Seq data in lymphoblastoid cell lines, we identified multiple loci with overlapping sense/antisense transcripts, of which one is expressed paternally and the other maternally. Using a sliding window approach, we searched for imprinted expression across the entire genome, identifying a novel imprinted putative lncRNA in 13q21.2. Overall, we identified 7 transcripts showing parental bias in gene expression which were not reported in 4 other recent RNA-Seq studies of imprinting. Conclusions: Our methods and data provide a robust and high-resolution map of imprinted gene expression in the human genome. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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