Imaging Human Immune Cell Infiltration in a Xenograft Graft-Versus-Host Disease Model
| العنوان: | Imaging Human Immune Cell Infiltration in a Xenograft Graft-Versus-Host Disease Model |
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| المؤلفون: | Jessica R. Ingram, Juanjo Cragnolini, Andrew M. Tager, Mohammad Rashidian, Christian Freund, Catharina H. M. J. Van Elssen, Vladimir Vrbanac, Hidde L. Ploegh |
| المصدر: | Blood. 128:5720-5720 |
| بيانات النشر: | American Society of Hematology, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Severe combined immunodeficiency, biology, business.industry, medicine.medical_treatment, Immunology, Cell Biology, Hematology, Hematopoietic stem cell transplantation, medicine.disease, Major histocompatibility complex, Biochemistry, Transplantation, Graft-versus-host disease, medicine.anatomical_structure, medicine, biology.protein, Cancer research, Bone marrow, Stem cell, business, Infiltration (medical) |
| الوصف: | Graft versus host disease (GvHD) is one of the major complications of hematopoetic stem cell transplantation (HSCT), and is characterized by massive alloreactive T cell infiltration into target organs and tissues. Currently, non-invasive tools to diagnose and follow up disease are lacking. Here we show non-invasive imaging of infiltrating immunocytes in a human xenograft model for chronic GvHD, using a single domain camelid-derived antibody fragment (VHH) specific for human Class II MHC products present on myeloid cells, B cells and activated T cells. NSG (NOD-scid gamma) mice reconstituted with human fetal thymus, bone marrow and liver (BLT mice) spontaneously develop a GvHD-like condition, characterized by alopecia, blepharitis and target organ infiltration by activated human T cells. In diseased animals, we show an increased PET signal in the liver, attributable to infiltration of activated Class II MHC positive T cells. Non-invasive imaging of specific immune infiltration and activation could thus be of importance for diagnosis and treatment evaluation of GvHD and holds promise for other diseases characterized by inflammation. Disclosures No relevant conflicts of interest to declare. |
| تدمد: | 1528-0020 0006-4971 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::2871f807add8a2cec1b89de8c3da64eeTest https://doi.org/10.1182/blood.v128.22.5720.5720Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........2871f807add8a2cec1b89de8c3da64ee |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15280020 00064971 |
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