The Vasculopathy in the Bone Marrow Microenvironment of Humanized Sickle Cell Mice Is Reversible By Blood Transfusion
| العنوان: | The Vasculopathy in the Bone Marrow Microenvironment of Humanized Sickle Cell Mice Is Reversible By Blood Transfusion |
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| المؤلفون: | Charles Y. Lin, Jina Ryu, Shin-Young Park, Yookyung Jung, Min Liu, Davide Melisi, Lucia De Franceschi, Leslie E. Silberstein, Alessandro Matte, Takashi Nagasawa, Wilson Anand, Eun Young Anna Han, Carmine Carbone |
| المصدر: | Blood. 134:2256-2256 |
| بيانات النشر: | American Society of Hematology, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Blood transfusion, biology, business.industry, medicine.medical_treatment, Growth factor, Immunology, Cell, Cell Biology, Hematology, medicine.disease, Biochemistry, Sickle cell anemia, medicine.anatomical_structure, Reticular cell, medicine, biology.protein, Stromal cell-derived factor 1, Bone marrow, Stem cell, business |
| الوصف: | Sickle cell disease (SCD) is a monogenic red cell disorder with high morbidity and mortality due to significant end-organ disease. Here we investigated for the first time the impact of SCD on the bone marrow (BM) vascular niche, which is critical for hematopoiesis. In SCD mice, we find a disorganized and structurally abnormal BM vasculature with highly tortuous arterioles and fragmented sinusoidal vessels, the latter filled with aggregates of sickle erythroid and myeloid cells. By in vivo imaging, sickle and control red blood cells exhibit significantly slow intravascular flow speeds in sickle cell BM but not in control BM. These abnormalities lead to increased ROS production in expanded erythroblast populations, and to up-regulation in the BM of HIF-1a and key growth factors required to support angiogenesis, e.g. VEGF-A, Ang1, Ang2. Moreover, increased levels of VCAM-1, a marker for vascular dysfunction, are detected. SCD also impacts the BM perivascular niche, i.e. the number of CXCL12-abundant reticular cells, is markedly decreased, and is associated with mobilization of hematopoietic stem/progenitor cells as well as mature leukocytes to the periphery. Strikingly, the distorted vascular network is completely reversed by blood transfusion, which highlights the plasticity of the BM vascular niche. Thus, we provide molecular and cellular features of the pathologic BM microenvironment, which suggest that the local hypoxia-induced angiogenic network combined with inflammatory vascular dysfunction is responsible for the vasculopathy. In addition, the findings may serve to develop new biomarkers and therapeutic targets of BM end-organ damage. Figure Disclosures No relevant conflicts of interest to declare. |
| تدمد: | 1528-0020 0006-4971 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::9f1dcd81cd9473fd7dd822a6bc1acbf0Test https://doi.org/10.1182/blood-2019-123532Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........9f1dcd81cd9473fd7dd822a6bc1acbf0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15280020 00064971 |
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