Phosphotyrosine profiling identifies the KG-1 cell line as a model for the study of FGFR1 fusions in acute myeloid leukemia
| العنوان: | Phosphotyrosine profiling identifies the KG-1 cell line as a model for the study of FGFR1 fusions in acute myeloid leukemia |
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| المؤلفون: | Valerie L. Goss, Cynthia Reeves, Michael J. Comb, Lana Popova, Brian J. Druker, Julie Nardone, Roberto D. Polakiewicz, Denise K. Walters, Joan MacNeill, John Edward Rush, Yi Wang, Ting-Lei Gu |
| المصدر: | Blood. 108:4202-4204 |
| بيانات النشر: | American Society of Hematology, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Small interfering RNA, Oncogene Proteins, Fusion, Immunology, Apoptosis, Biology, Models, Biological, Biochemistry, chemistry.chemical_compound, Cell Line, Tumor, Proto-Oncogene Proteins, STAT5 Transcription Factor, medicine, Humans, Receptor, Fibroblast Growth Factor, Type 1, RNA, Small Interfering, STAT5, Genetics, ABL, Myeloid leukemia, Tyrosine phosphorylation, Cell Biology, Hematology, medicine.disease, Molecular biology, Leukemia, Myeloid, Acute, stomatognathic diseases, Leukemia, chemistry, Cell culture, biology.protein, Phosphorylation |
| الوصف: | The 8p11 myeloproliferative syndrome (EMS) is associated with translocations that disrupt the FGFR1 gene. To date, 8 fusion partners of FGFR1 have been identified. However, no primary leukemia cell lines were identified that contain any of these fusions. Here, we screened more than 40 acute myeloid leukemia cell lines for constitutive phosphorylation of STAT5 and applied an immunoaffinity profiling strategy to identify tyrosine-phosphorylated proteins in the KG-1 cell line. Mass spectrometry analysis of KG-1 cells revealed aberrant tyrosine phosphorylation of FGFR1. Subsequent analysis led to the identification of a fusion of the FGFR1OP2 gene to the FGFR1 gene. Small interfering RNA (siRNA) against FGFR1 specifically inhibited the growth and induced apoptosis of KG-1 cells. Thus, the KG-1 cell line provides an in vitro model for the study of FGFR1 fusions associated with leukemia and for the analysis of small molecule inhibitors against FGFR1 fusions. |
| تدمد: | 1528-0020 0006-4971 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::84129a293a46b962ed645c894addabd5Test https://doi.org/10.1182/blood-2006-06-026666Test |
| رقم الانضمام: | edsair.doi.dedup.....84129a293a46b962ed645c894addabd5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15280020 00064971 |
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