Enhancing saccharification of wheat straw by mixing enzymes from genetically-modified Trichoderma reesei and Aspergillus niger
| العنوان: | Enhancing saccharification of wheat straw by mixing enzymes from genetically-modified Trichoderma reesei and Aspergillus niger |
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| المؤلفون: | Jiang, Yanping, Duarte, Alexandra Vivas, van Den Brink, Joost, Wiebenga, Ad, Zou, Gen, Wang, Chengshu, de Vries, Ronald P., Zhou, Zhihua, Benoit, Isabelle, Sub Molecular Plant Physiology, Sub Molecular Microbiology, Molecular Plant Physiology |
| المساهمون: | Sub Molecular Plant Physiology, Sub Molecular Microbiology, Molecular Plant Physiology |
| المصدر: | Biotechnology Letters, 38(1). Springer Netherlands Biotechnology Letters, 38(1), 65-70. Springer Netherlands Biotechnology Letters |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Trichoderma reesei, 030106 microbiology, Bioengineering, Genetically-modified strains, Cellulase, Saccharification, Applied Microbiology and Biotechnology, Fungal Proteins, 03 medical and health sciences, Hydrolysis, Enzymatic hydrolysis, parasitic diseases, Biomass, Food science, Triticum, Trichoderma, Fungal protein, Organisms, Genetically Modified, biology, Chemistry, Aspergillus niger, food and beverages, General Medicine, Wheat straw, Straw, biology.organism_classification, Saccharum, Genetically modified organism, Original Research Paper, Agronomy, Mutation, biology.protein, Biotechnology |
| الوصف: | Objectives To increase the efficiency of enzymatic hydrolysis for plant biomass conversion into renewable biofuel and chemicals. Results By overexpressing the point mutation A824 V transcriptional activator Xyr1 in Trichoderma reesei, carboxymethyl cellulase, cellobiosidase and β-d-glucosidase activities of the best mutant were increased from 1.8 IU/ml, 0.1 IU/ml and 0.05 IU/ml to 4.8 IU/ml, 0.4 IU/ml and 0.3 IU/ml, respectively. The sugar yield of wheat straw saccharification by combining enzymes from this mutant and the Aspergillus niger genetically modified strain ΔcreA/xlnRc/araRc was improved up to 7.5 mg/ml, a 229 % increase compared to the combination of wild type strains. Conclusions Mixing enzymes from T. reesei and A. niger combined with the genetic modification of transcription factors is a promising strategy to increase saccharification efficiency. Electronic supplementary material The online version of this article (doi:10.1007/s10529-015-1951-9) contains supplementary material, which is available to authorized users. |
| وصف الملف: | image/pdf |
| اللغة: | English |
| تدمد: | 0141-5492 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::909d7aa38b453b4e4a159125edf1a1edTest https://hdl.handle.net/20.500.11755/c804c93c-4f9c-4717-bb2a-554718477aa6Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....909d7aa38b453b4e4a159125edf1a1ed |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 01415492 |
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