دورية أكاديمية
Transformation of yeast using calcium alginate microbeads with surface-immobilized chromosomal DNA
| العنوان: | Transformation of yeast using calcium alginate microbeads with surface-immobilized chromosomal DNA |
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| المؤلفون: | Atsushi Mizukami, Eiji Nagamori, Yukiko Takakura, Sachihiro Matsunaga, Yoshinobu Kaneko, Shin’ichiro Kajiyama, Satoshi Harashima, Akio Kobayashi, Kiichi Fukui |
| المصدر: | BioTechniques, Vol 35, Iss 4, Pp 734-740 (2003) |
| بيانات النشر: | Future Science Ltd |
| سنة النشر: | 2003 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Biology (General), QH301-705.5 |
| الوصف: | Yeast artificial chromosomes (YACs) are useful cloning vectors that have the capacity to carry large DNA inserts. The largest barrier to using such large DNA molecules in transformation experiments has been their physical instability in solution. We developed a new method of transforming yeast using chromosome-sized DNA. The method uses calcium alginate microbeads to immobilize high-density yeast chromosomal DNA. Chromosomal DNA immobilized on microbeads is physically stabilized compared with naked chromosomal DNA. The microbead-mediated transformation performed well, not only with respect to the transformation frequency with large DNA molecules (>100 kb) but also in successful transformation using split chromosome DNA that exceeded 450 kb. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1940-9818 0736-6205 |
| العلاقة: | https://doaj.org/toc/0736-6205Test; https://doaj.org/toc/1940-9818Test; https://doaj.org/article/74156745bd474372b768953ce1efd8e1Test |
| DOI: | 10.2144/03354st03 |
| الإتاحة: | https://doi.org/10.2144/03354st03Test https://doaj.org/article/74156745bd474372b768953ce1efd8e1Test |
| رقم الانضمام: | edsbas.F747E584 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19409818 07366205 |
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| DOI: | 10.2144/03354st03 |