دورية أكاديمية
Establishment of a pseudovirus neutralization assay based on SARS-CoV-2 S protein incorporated into lentiviral particles
| العنوان: | Establishment of a pseudovirus neutralization assay based on SARS-CoV-2 S protein incorporated into lentiviral particles |
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| المؤلفون: | Sheng Wang, Lizhen Liu, Can Wang, Ziqiang Wang, Xuhua Duan, Gang Chen, Hu Zhou, Hong Shao |
| المصدر: | Biosafety and Health, Vol 4, Iss 1, Pp 38-44 (2022) |
| بيانات النشر: | Elsevier, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Infectious and parasitic diseases LCC:Public aspects of medicine |
| مصطلحات موضوعية: | COVID-19, SARS-CoV-2 variants, Pseudovirus, Neutralizing antibody, RBD, Infectious and parasitic diseases, RC109-216, Public aspects of medicine, RA1-1270 |
| الوصف: | The coronavirus disease 2019 (COVID-19) is still causing a wide range of infections and deaths due to the high variability of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Therefore, it is necessary to establish a reliable and convenient pseudovirus-based neutralization assay to develop drug targeted variants of SARS-CoV-2. Based on the HIV-1 backbone, we generated a high titer luciferase (Luc)-expressing pseudovirus packaging system. Three dominant S mutant substitution pseudovirus were also established and identified compared to wide type in hACE2-overexpressing HEK-293T cells (293T-ACE2 cells). Compared to serine protease inhibitor camostat mesylate, the cysteine protease inhibitor E-64d could significantly block all SARS-CoV-2 mutant S pseudovirus infection in 293T-ACE2 cells. Furthermore, the neutralization ability of two antibodies targeted receptor-binding domain (RBD) of SARS-CoV-2 spike protein (S) was evaluated, which showed different inhibition dose–effect curves among four types of S pseudovirus. Overall, we developed a pseudovirus-based neutralization assay for SARS-CoV-2, which would be readily adapted to SARS-CoV-2 variants for evaluating antibodies. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2590-0536 |
| العلاقة: | http://www.sciencedirect.com/science/article/pii/S2590053621001269Test; https://doaj.org/toc/2590-0536Test |
| DOI: | 10.1016/j.bsheal.2021.12.006 |
| الوصول الحر: | https://doaj.org/article/06e2e75a5d414b2fb8931271ea4345cbTest |
| رقم الانضمام: | edsdoj.06e2e75a5d414b2fb8931271ea4345cb |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 25900536 |
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| DOI: | 10.1016/j.bsheal.2021.12.006 |